Metabolic Biotinylation of Recombinant Proteins

Recombinant protein is a protein obtained by applying recombinant DNA/RNA technology. The production of recombinant proteins in vitro mainly includes four major systems: prokaryotic protein expression, mammalian cell protein expression, yeast protein expression and insect cell protein expression. Compared with natural proteins, recombinant proteins have batch-to-batch consistency and have better performance. The emergence of recombinant proteins has accelerated the breakthroughs in protein structure and function, and cell pathways have been in the field of life science research. The avidin-biotin system is an important tool in biomedicine and can be widely used in immunolocalization, bioimaging, nucleic acid imprinting and protein labeling. Metabolic biotinylation of recombinant proteins allows rapid protein labeling. At the same time, high-efficiency biotinylation is achieved at the fixed site of the protein, which is conducive to the binding of the targeted part of the biotin protein.

The Services

Protein biotinylation is widely used in the fields of molecular biology and biomedicine. Based on the high-strength interaction between biotin and avidin, biotin can be covalently attached to reagents in vitro and thus can be used to label biomolecules. Recombinant proteins are formed by recombining DNA or RNA through high-tech biotechnology. Fast and efficient biotin labeling of recombinant proteins is helpful for specific site labeling, which is of great significance for targeted binding. Alfa Chemistry researchers have been engaged in biotinylation-related research for a long time, and the related services they can provide include but are not limited to:

• Purification of recombinant proteins
• DNA transfection in living cells
• Western blot analysis
• Metabolic biotinylation service for recombinant proteins
• Solution design and update

Biotinylated recombinant protein

Production of biotinylated recombinant proteins is mediated due to simple plasmid expression vectors. Transgene expression products and biotinylation from these plasmids are sensitive. Biotinylation of plasmids does not require additional biotin, and increased levels of biotin affect protein biotinylation. Biotin is a typical vitamin capable of purifying and metabolizing biotinylated proteins in mammals. Biotin is usually stored in humans and animals, so it is a very safe reagent during purification.

Service features and benefits

The researchers of Alfa Chemistry have been committed to biological research for a long time, have maintained long-term close cooperation with the world's leading companies, mastered advanced technologies and methods, and can perform efficient biotinylation of recombinant proteins in vivo or in vitro.

• Controllable biotinylation at specific sites
• Biotin labeling is uniform
• Small batch-to-batch variance

Advantages of biotinylation labeling

• The small size of biotin reduces any interference in proteins and enables multiple biotins to bind to proteins
• The unique structural advantages of biotin enable rapid chemical modification without affecting functional properties

Alfa Chemistry can provide professional biotinylation services for recombinant protein metabolism, and can carry out efficient design and development to serve customers around the world. Our researchers have many years of experience and can fully meet the needs of customers. If you are interested in our services, please contact us immediately.

Reference:

1. M. Brandon. P.; et al. Metabolic biotinylation of recombinant proteins in mammalian cells and in mice. MOLECULAR THERAPY. 2000, 1(1): 96-104.

※ It should be noted that our service is only used for research, not for clinical use.