Enzymatic RNA Biotinylation

Biotinylation, the process of covalently attaching biotin to biomolecules, can be accomplished chemically or enzymatically. Enzymatic biotinylation was highly consistent, while also supporting the predetermined orientation of avidin or streptavidin. RNA is involved in gene regulation processes in organisms (such as chromatin remodeling and transcriptional activation) and is also involved in many human diseases (cancer and neurodegenerative diseases). Efficient and accurate characterization of the actions of RNA and other biomolecules is critical for understanding RNA biology and developing novel therapeutic strategies. Alfa Chemistry has a professional biotech team, familiar with the methods and strategies centered on biotinylation, and is committed to providing customers with professional enzymatic RNA biotinylation.

The Services

Biotinylation can be accomplished chemically or enzymatically. RNA is involved in gene regulation of organisms and has a direct relationship with many diseases, so it is of great significance for enzymatic RNA biotinylation. Alfa Chemistry has long focused on biotinylation-related services, including:

• Sequencing of enzymatic RNA biotinylation
• Enzymatic RNA biotinylation data analysis

About RNA and biotinylation

Ribonucleic acid (abbreviated RNA), found in all living cells, is involved in regulating gene expression. Compared to DNA, RNA has a similar structure, but RNA is single-stranded. The molecular backbone of RNA is composed of alternating phosphate groups and sugar ribose sugars. RNAs in cells include: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). RNA transcripts bind to proteins and play critical roles in RNA metabolism, transport, and function. Enzymatic biotinylation is a strictly specific reaction that requires specific recognition of the target sequence.

Our team strength and support

Our team is composed of an experienced researcher who has been engaged in biotinylation research for many years and can provide relevant support. We also cooperate with the world's leading teams, master the leading biotechnology, and provide customers with satisfactory RNA sequencing and technical support.

Alfa Chemistry will focus on biotinylation services for a long time in the future, including the detection and data analysis of enzymatic RNA biotinylation. If you are interested in our services, please contact us immediately, we always look forward to hearing from you.

Reference:

1. Kayla. N.B .; et al. Enzymatic RNA Biotinylation for Affiffiffinity Purifification and Identifification of RNA−Protein Interactions. ACS Chem. Biol. 2020, 15: 2247-2258.

※ It should be noted that our service is only used for research, not for clinical use.