Promiscuous Protein Biotinylation

Protein biotinylation can usually be carried out by chemical and biological means. Chemical biotinylation is usually the modification of amino groups of proteins with biotin-N-hydroxysuccinimide or similar acylating agents. Enzymatic biotinylation usually modifies small amounts of proteins, mainly by biotin-dependent carboxylases and decarboxylases. The biotin-avidin/streptavidin interaction is one of the strongest known, allowing several robust protocols for sensitive detection of biotinylated proteins. High-quality biotinylated proteins are currently a major challenge, often encountered with inadequate labeling and large batch-to-batch variability. The way of biotinylation will significantly affect the results, and each protein is unique, so it is of great significance to design a unique and efficient biotinylation strategy. Alfa Chemistry can provide efficient biotinylation strategies to achieve more efficient biotinylation services, showing great application potential in the field of proteomics.

The Services

Alfa Chemistry is a professional biotinylation supplier, which can develop and provide a series of protein biotinylation services. Efficient biotinylation strategy, capable of strict quality control strategy to ensure small batch-to-batch variation. We have long been focusing on advanced protein biotinylation services, including but not limited to:

• Promiscuous in vivo biotinylation

The biological activity of biotinylated protein depends on the structure of the protein itself and the way of biotinylation. For special protein samples, a reasonable biotinylation strategy needs to be customized to achieve efficient biotinylation reactions.

• Promiscuous in vitro biotinylation

Biotin ligase can be used to catalyze biotinylation reactions and is also an inhibitor of biotin induction. A common biotin ligase is the BirA enzyme in E. coli that recognizes protein interactions.

Our team strength and advantages

At Alfa Chemistry, we are always innovating, keeping pace with the times and insisting on introducing new technologies to meet the needs of our customers. We have a dedicated team working on a wide range of biotinylation solutions and strategies. We have experienced scientific researchers who will participate in the customer's biotinylation service throughout the process, and can support the detection of various samples to meet the needs of customers. The advantages of our services are as follows:

• We focus on efficient biotinylation solutions, which can be applied to the biotinylation of specific sites of samples
• Support biotinylation detection project management, can support professional data analysis
• Professional biotinylation platform, perfect service system, strict quality control system
• Innovative technical support to meet customer needs with precise biotinylation services

Figure 2. Efficient and accurate biotinylation service, perfect service system, optimized service process, insisting on meeting customer needs

Why choose us?

• Broad range of biotinylation solutions and strategies
• Professional team, providing accurate data analysis
• Optimized service process
• Responsive biotinylation project
• Professional biotechnology team, pioneering biotinylation service
• Support global payments

Alfa Chemistry can provide efficient promiscuous biotinylation strategies, including in vivo and in vitro promiscuous biotinylation, to meet customer needs. If you are interested in our services, please contact us immediately.

Reference:

1. Eunjoo. C. R.; et al. Promiscuous protein biotinylation by Escherichia coli biotin protein ligase. Protein Science. 2008, 29(144): 3043-3050.

※ It should be noted that our service is only used for research, not for clinical use.