Avidin-biotin Complexes in Bioanalytical Applications

Avidin-biotin Complexes in Bioanalytical Applications

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Avidin-biotin Complexes in Bioanalytical Applications

Avidin-biotin Complexes in Bioanalytical Applications

Unlike other biomolecular interactions (antigen-antibody interactions, enzyme-substrate interactions, hormone-receptor interactions, and biotin-avidin interactions), biotin-avidin interactions are currently the most One of the strong non-covalent interactions. The robust biotin-avidin complex system can be used as an efficient research tool in the field of biological research, including biological diagnosis and gene therapy. Based on the easy modification of biotin, functional modification can be carried out, which is one of the key directions of current research. Alfa Chemistry can develop high-efficiency biotin-avidin complex systems, and strive to expand the application in the field of biomolecules, including: affinity chromatography, immunodiagnosis, gene probes and localization observation. We have an advanced quality assurance system and can strictly abide by industry standards. Throughout the service process, our researchers will participate in it and provide customers with feasible help and advice.

The Services

Alfa Chemistry has experienced experts with many years of experience in the field of biological research, and can provide customized services according to customer needs. We adhere to the purpose of innovation and constantly meet the needs of customers. The related services we can provide include but are not limited to:

The separation principle of affinity chromatography is based on the immobilization of affinity biomolecules on an insoluble matrix, and another molecule can be purified using high-efficiency affinity energy. Ligands immobilized on matrices can be used to purify antibodies, enzymes, hormones, and other biomolecules through interactions.

Immunoassay is an important biological analysis method, which can be widely used in biopharmaceutical analysis, clinical diagnosis and environmental detection and other fields. Immunoassays can be used to quantify the concentration of a specific analyte in solution, using antibodies or antigens as biorecognizers.

Gene-specific probes are produced from specific mRNA by reverse transcriptase, which synthesizes a complementary DNA copy (cDNA) from the mRNA. Traditional gene probes are synthesized from radioactive phosphate bases.

Taking advantage of the strong interaction between biotin and avidin, biotin and biotin derivatives can be combined with glycoproteins on the cell surface, and then labeled with avidin, so as to realize the localization of probes at the cellular or subcellular level.

Our service advantages

  • Professional quality control platform, strict implementation of industry norms
  • Experienced team support, adhere to high-quality service to meet customer needs
  • Scalable biotin-avidin platform
  • The world's leading cooperative team maintains a close cooperative relationship

Our service support and capabilities

Alfa Chemistry's research team understands customers' needs and aims to provide customers with efficient and fast services. Our researchers participate in the customer service throughout the process, provide a comprehensive project plan, and will promptly contact the customer with the project progress, and we will serve the customer wholeheartedly.

Alfa Chemistry has an advanced team and has established a complete quality control system to provide customers with high-quality biodiagnostic services based on the biotin-avidin system. If you are interested in our services, please contact us immediately.

References:

  1. Péter. H.; et al. Functionalized liposomes loaded with siRNAs targeting ion channels in effector memory T cells as a potential therapy for autoimmunity. Biomaterials. 2013. 34(38): 10249-10257.
  2. Meir. W.; et al. The Avidin-biotin complex in bioanalytical applications. ANALYTICAL BIOCHEMISTRY. 1988, 171: 1-32.

※ It should be noted that our service is only used for research, not for clinical use.