Biotinylated Expression, Purification and Characterization

In biochemistry, biotinylation refers to the process of covalently attaching biotin to proteins, nucleic acids, or other biological macromolecules. The biotinylation reaction is fast, efficient and insensitive to interference (unaffected by heat, pH and proteolysis). Biotinylation methods include chemical biotinylation and enzymatic biotinylation. BirA is a biotin ligase that catalyzes the covalent attachment of biotin to specific lysine residues of BAP fused to recombinant proteins. Biotinylation of specific sites can be achieved through the expression of recombinant proteins. The presence of endogenous BirA in E. coli improves purification and near homogeneity, and co-expression increases biotinylation efficiency. Alfa Chemistry provides a wide range of biotinylation-related services, improves service provision, focuses on a wide range of biotinylation strategies, and insists on meeting customer needs with high-quality services.

The Services

Alfa Chemistry has a professional biotechnology team that can provide a complete and complete service system. We provide efficient biotinylation expression, detection and purification services including but not limited to:

• Construction of bacterial expression vectors
• Expression and purification of recombinant proteins
• Western blot analysis
• Determination of biotinylation efficiency
• Enzyme assays, protein and spectroscopic assays
• Construction of gene product expression vector
• Biotinylation detection, expression
• Site-specific biotinylation

Efficient purification of biotinylation is of great significance to the study of biology

Biotinylation refers to the process of covalently attaching biotin to biomacromolecules (proteins or nucleic acids, etc.), and is involved in important physiological processes. The biotinylation reaction has the advantages of fast response, high efficiency, and strong anti-interference ability. Proteins participate in important processes of organisms and are the basis of life activities. With the deepening of the fields of molecular biology, structural biology and genomics, more and more biotinylation studies are carried out based on essential research. The most basic requirement for biotinylation is highly purified, biologically active and stable proteins. It also requires accurate characterization of protein interactions, which is of great significance for a detailed understanding of molecular biology.

The significance

• Study the essence of life, understand the laws of life activities, and reveal the significance of biotinylation
• Genetic engineering requires modification of biotinylation reactions with physiological functions to improve physiological activity, enzyme activity and stability
• Accurate study of biotinylation is of great significance for the study of protein interactions

Our team support

• We have a professional biotechnology team to provide customers with precise biotinylation technical support in order to be familiar with biological performance
• We keep pace with the times, insist on innovation, and update the biotinylation platform to meet the needs of customers

Alfa Chemistry is committed to a wide range of biotinylation solutions and strategies, can carry out technical innovation according to customer needs, and insist on meeting customer needs. If you are interested in our services, please contact us immediately.

Reference:

1. Gaurav. B.; et al. Expression, purification and characterization of in vivo biotinylated dengue virus envelope domain III based tetravalent antigen. Protein Expression and Purification. 2010, 74: 99-105.

※ It should be noted that our service is only used for research, not for clinical use.