Biotin Reagents for Antibody Pretargeting

In recent years, with the efficient research on tumors, various research methods have been developed. Target-mediated radionuclide intervention has emerged as a powerful tool for noninvasive molecular imaging and cancer therapy. Based on the high affinity of antibodies to target antigens, yes antibodies have become a typical carrier, which can be designed to achieve cancer therapy through pre-targeting. In a pre-targeting approach, the tumor-targeting selectivity of the antibody can be preserved and the delivery of the radioactive nucleic acid is diverted to another molecule. In many studies, the molecules were radiolabeled biotin derivatives or radiolabeled avidin or streptavidin molecules. Due to the strong interaction between biotin and avidin or streptavidin, it is widely used in the field of pretargeting therapy. Compared with traditional chemotherapy, targeted therapy for tumors has a more precise therapeutic effect and has little effect on normal cells. More and more studies are being made on the development and design of drugs for targeted therapy, so that the drugs only act on specific target tumor cells, so the specificity is strong, the therapeutic effect is obvious, and the adverse reactions are milder.

The Services

Antibodies are highly efficient carriers in the field of tumor therapy. Based on their high specificity and high binding ability to target antigens, they are widely used in biological therapy. Alfa Chemistry researchers have been engaged in related research for many years, and the services that can be provided based on biotinylated antibody pre-targeting include but are not limited to:

• Biotinylated antibody-based pre-targeting design and development
• Material characterization
• Cytotoxicity assay
• Efficient production of biotinylated targeting antibodies
• Design and development of pretargeted alpha particle therapies
• Biotin system interaction test

About pretargeted therapy

Targeted therapy provides reliable hope for efficient treatment of tumors. Pretargeting strategies have played an important role in addressing the high toxicity of radioimmunotherapy. Central to pretargeting therapy is the concept of decoupling and administering the targeting carrier and cytotoxic agent separately. Pre-targeted therapy has a more precise therapeutic effect and can reduce side effects. In recent years, pretargeting strategies have been widely used in different types of cancer therapy. The pre-targeting process is as follows:

1. Targeting carrier for targeted binding is injected

2. When the targeting vector accumulates at the target site, a complementary radiolabeled small molecule is used

3. The targeting carrier will combine with radioactive substances to form radioactive conjugates

The use of pre-targeting methods is more precise than other current treatment methods and will have less impact on normal cells. With the technology of anytime, the scope of application of the pre-targeting strategy will be expanded, and it is expected to become an effective tool for other tumor treatments.

Figure 2. The pretargeting concept

Development and application of biotinylated antibody pre-targeting strategy

Pretargeting has proven to be a feasible and promising therapeutic strategy. Antibodies have been considered as a typical high-efficiency carrier of targeted therapy, and are expected to have wider applications in theranostics. Based on the high affinity between biotin and avidin, this system is the most widely used in research. Due to its high affinity, avidin can achieve a high degree of tumor uptake, which makes the formation of this system have great application potential in the field of tumor research. Although pre-targeting strategies have a wide range of applications, pre-targeting has not yet been implemented clinically and remains a very promising prospect for future development.

Alfa Chemistry insists on meeting customers' needs with high-quality services, focusing on the synthesis of biotinylated preparations for a wide range of applications. If you are interested in our services, please contact us immediately.

References:

1. Scott. W.; et al. Biotin reagents for antibody pretargeting. 3. synthesis, radioiodination, and evaluation of biotinylated starburst dendrimers. Bioconjugate Chem. 1998, 9: 813-825.
2. Marjolein. V.; et al. Applications of pretargeting. Pharmaceutics. 2019, 11: 434.

※ It should be noted that our service is only used for research, not for clinical use.

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