Promiscuous In Vitro Biotinylation

Biotinylated proteins can be purified by high affinity avidin or streptavidin. Biotinylation can be carried out chemically and biologically. Biotin ligase can be used to catalyze biotinylation reactions and is also an inhibitor of biotin induction. A common biotin ligase is the BirA enzyme in E. coli that recognizes protein interactions. The way of biotinylation will significantly affect the results, and each protein is unique, so it is of great significance to design a unique and efficient biotinylation strategy. The BirA enzyme is a robust protein that retains its essential activity even at room temperature. Since its establishment, Alfa Chemistry has insisted on being customer-centric, and can provide customized services according to customer needs. Alfa Chemistry has experienced researchers who can select suitable promiscuous bioreceptors for in vitro biotinylation reactions. We provide efficient in vitro promiscuous biotinylation service strategies that are widely applicable to protein purification and immobilization.

The Services

Alfa Chemistry researchers work on promiscuous biotinylation strategies and protocols for in vitro and in vivo use. Alfa Chemistry has a professional biotinylation team that can provide high-quality biotinylation strategies designed to meet a wide range of needs. The services we can provide include but are not limited to:

• Testing of promiscuously biotinylated proteins
• Professional in vitro promiscuous biotinylation service
• Professional data analysis
• Determination of the degree and efficiency of biotinylation
• Quantitative analysis

What can we provide?

• Professional biotinylation system
• Optimized service process
• Self-developed production route
• Efficient in vitro promiscuous biotinylation assays
• Professional in vitro promiscuous biotinylation strategies and solutions
• Vector construction
• Efficient quality control platform
• Global biotinylation services

About biotin ligase

The binding between biotin and streptavidin or avidin is one of the strongest known non-covalent biological interactions and is widely used in biological research and biotechnology. Protein labeling with biotin is an efficient method for protein capture, immobilization and functionalization of conjugated biomolecules. BirA enzyme is an E. coli biotin ligase that performs site-specific biotinylation of lysine side chains within the 15 amino acid acceptor peptide. BirA enzyme is an important reagent for biotinylation reactions, which can recognize biotinylated proteins at specific residues in the sequence.

Our team strength and support

• Professional biotechnology team, providing efficient in vivo promiscuous biotinylation strategies to meet customer needs
• Cooperate with the world's leading team, keep pace with the times, and insist on innovation
• Equipped with advanced equipment, providing professional technology and support
• Professional quality control platform, strictly control service quality

Alfa Chemistry provides professional in vitro promiscuous biotinylation services, focusing on high-quality biotinylation solutions and strategies, and supporting responsive biotinylation projects. If you are interested in our services, please contact us immediately.

References:

1. Eunjoo. C. R.; et al. Promiscuous protein biotinylation by Escherichia coli biotin protein ligase. Protein Science. 2008, 29(144): 3043-3050.
2. Li, Y.F.; et al. Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation. Protein Expr Purif. 2012, 82(1): 162-167.

※ It should be noted that our service is only used for research, not for clinical use.