In Vitro Protein Biotinylation

In biochemistry, biotinylation refers to the process of covalently attaching biotin to proteins, nucleic acids, or other biomolecules. Biotinylated molecules can interact with avidin or streptavidin through biotin, which is not affected by heat, pH and proteolysis. In vitro protein biotinylation can be divided into in vitro protein biotinylation and in vitro enzymatic protein biotinylation, which can be widely used in the fields of biochemistry, cell biology and biological detection. Alfa Chemistry specializes in providing efficient biotinylation services, and strives to optimize the service system to meet customer needs. Our scientific researchers have many years of experience in the field of biotinylation, and can provide customized biotinylation services according to customer needs. We can design reasonable and perfect in vitro biotinylation strategies for different protein samples to meet the needs of customers.

The Services

Alfa Chemistry is committed to providing efficient in vitro biotinylation services for the world, providing comprehensive in vitro biotinylation strategies to meet customer needs. Services we can provide include but are not limited to:

• In vitro enzymatic biotinylation of proteins

Enzymatic protein biotinylation can only be attached to proteins by biotin ligase, which can be widely used in biochemistry, cell biology and bioassay techniques. Biotin ligase can link biotin to protein with high specificity, and can realize protein biotinylation in vivo or in vitro.

• In vitro chemical biotinylation of proteins

Chemical biotinylation is one of the important biotinylation methods, which can combine biotin with other biomolecules to form a stable structure. Compared with enzymatic biotinylation, chemical biotinylation requires less specificity and has a wider range of applications.

Biotinylated protein

Biotinylation is the process of attaching biotin to protein biomolecules for efficient protein detection and purification. The affinity of biotin-avidin is at least one million times higher than the binding force of antigen-antibody, and it is the substance with the strongest affinity found in nature so far. The biotin-avidin/streptavidin system is widely used in immunodiagnosis and biological testing. Biotin is easy to combine with protein in the form of covalent bond, and usually can be specifically bound by biological enzymes for biotinylation reaction. The biotinylation reaction amplifies the signal for efficient bioassays.

Factors to consider for protein biotinylation reagents:

• Solubility: The solubility of reagents that can be biotinylated can greatly affect the ability of the protein
• The length of the spacer arm: the binding capacity between biotin and protein is affected by the length of the spacer arm
• Reversibility: biotin-avidin/streptavidin interaction can be achieved through reversibility for recovery and purification

Figure 2. Protein biotinylation reagents need to take into account many factors, such as solubility, wall length and reversibility

Advantages of in vitro biotinylated proteins

• Quality control: Each biotinylated protein will undergo a strict activity assay to ensure that the biotinylated protein does not affect the activity of the protein.
• Rich targets: We can customize according to the needs of customers and provide efficient biotinylation services

Alfa Chemistry focuses on high-quality biotinylation solutions and strategies, and establishes a complete biotin system to meet customer needs. If you are interested in our services, please contact us immediately.

References:

1. Petri. S.; et al. In vitro enzymatic biotinylation of recombinant Fab fragments through a peptide acceptor tail. Bioconjugate Chem. 1998, 9(6): 725-735.
2. Marta. F.S.; et al. Protein−protein interaction detection in vitro and in cells by proximity biotinylation. J. Am. Chem. Soc. 2008, 130(29): 9251-9253.
3. Wilchek. M.; et al. Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein—protein and protein-ligand interaction. Immunol. Lett. 2006, 103, 27-32.

※ It should be noted that our service is only used for research, not for clinical use.