Enzymatic Thiol Biotinylation

Biotin, also known as vitamin B7, is a cofactor for the enzyme carboxylase required for fatty acid synthesis. Biotin can be covalently linked to proteins, polypeptides or nucleic acids by chemical or enzymatic means. The biotin-streptavidin system binds biotin to proteins. Currently, the use of state-of-the-art technologies is to capture protein networks and subcellular microenvironments in cells and organisms. Using biotin ligase, rapid protein biotinylation can be achieved. Researchers have successfully explored thiol-cleavable biotin as an alternative to elution of biotinylated proteins from streptavidin-coated beads for chemical biotinylation and biotin ligase-based proximity labeling. Alfa Chemistry has a high-quality biotechnology team that has been engaged in biotinylation research for a long time. Our researchers have been engaged in biotinylation research for many years, and can provide customized services according to customer needs, continuously optimize the service system, insist on innovation, and Better service to meet customer needs.

The Services

Biotinylation is the covalent attachment of biomolecules to proteins, polypeptides or nucleic acids, either chemically or enzymatically. Compared with chemical methods, enzymatic methods are highly recognizable and consistent. Alfa Chemistry focuses on providing customers with efficient biotinylation services. The enzymatic thiol biotinylation that we can provide includes but is not limited to:

• Thiol-cleavable biotin modification
• Development of thiol-cleavable biotin for chemical labeling

About thiol biotinylation

Thiols cleave biotin and serve as a near-labeled substrate for living cells. Biotin ligase can be used to be engineered into the inner mitochondrial membrane facing the mitochondrial matrix. A significant reduction in the digestion efficiency of trypsin can be achieved by thiol-cleavable biotinylation, and thiol-cleavable biotin can achieve high spatial resolution within organelles.

Alfa Chemistry is committed to providing biotinylation to serve customers worldwide with competitive price and quality. If you need, please feel free to contact us.

Reference:

1. Li. H.R .; et al. Thiol-cleavable biotin for chemical and enzymatic biotinylation and its application to mitochondrial turboID proteomics. J. Am. Soc. Mass Spectrom. 2021, 32(9): 2358-2365.

※ It should be noted that our service is only used for research, not for clinical use.