Avidin-biotin Complexes for Gene Probes

Gene probes can be produced in a variety of ways and fall into three types: gene-specific probes, oligonucleotide probes, and polymorphism probes. Gene-specific probes are produced from specific mRNA by reverse transcriptase, which synthesizes a complementary DNA copy (cDNA) from the mRNA. Traditional gene probes are synthesized from radioactive phosphate bases. Gene probes can be used in dot blot hybridization, where serially diluted DNA samples are preserved on DNA-binding membranes and complementary radiolabeled probes are in vitro, so that the amount of radioactive signal is directly proportional to the amount of target DNA present. The development of gene probes that recognize various DNA polymorphisms in non-coding sequences can realize the identification of abnormal genes and gene products at the molecular level. In recent years, biotin has been introduced into DNA probes, which are analyzed using appropriate conjugates containing avidin or streptavidin.

The Services

Alfa Chemistry insists on providing customers with efficient and accurate services, and strives to expand the application of the biotin-avidin system in the field of gene probes. The services it can provide include but are not limited to:

• Design and preparation of biotin-avidin system
• Biotin-avidin complex for gene probes
• Professional data analysis and comparison
• Professional gene probe labeling
• Solutions for biotin-avidin complex development

Classification of gene probes

• Genomic DNA Probe
• cDNA probe
• RNA probe

What can we provide?

• Professional gene probe analysis and labeling
• Professional gene probe preparation and design
• Sensitivity testing of probes

Our team support and capabilities

Alfa Chemistry has an advanced biotechnology team with many years of experience in related fields, capable of designing and developing the preparation and development of gene probes based on the biotin-avidin system. Over the years, our research team has insisted on being customer-centric, providing customized services according to the needs of customers, and insisting on providing efficient and accurate services related to gene probes based on the biotin-avidin system.

Quality control

• Special purification process, the purity of the probe is high
• Strictly implement industry norms, monitor in real time during the customization process, and have strong traceability between batches
• Provide responsive technical support, such as synthesized sequence, purification method, molecular weight and quantity, etc.
• Perfect custom synthesis system, experienced expert team and support

Features

Gene probes based on biotin-avidin can specifically recognize target molecules and can directly detect nucleic acid fragments of markers. Gene probes based on biotin-avidin have the characteristics of strong specificity, high sensitivity, speed and accuracy, and have great application potential in molecular diagnosis, tumor research, medical identification and other fields.

Alfa Chemistry can use the biotin-avidin system in the field of efficient gene probes to meet the needs of customers. If you are interested in our services, please contact us immediately.

Reference:

1. Meir. W.; et al. The Avidin-biotin complex in bioanalytical applications. ANALYTICAL BIOCHEMISTRY. 1988, 171: 1-32.

※ It should be noted that our service is only used for research, not for clinical use.

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