In Vitro and In Vivo Protein Biotinylation

Biotin is a naturally occurring metabolic enzyme factor that exhibits activity through the action of specific protein-biotin ligases. The physiological functions of organisms are mainly controlled and regulated by intracellular proteins. The biotinylation reaction of protein can be carried out by enzymatic biotinylation and chemical biotinylation. The binding between biotin and streptavidin or avidin is one of the strongest known interactions, characterized by rapid binding and high specificity. Labeling purified proteins with biotin is an efficient method for protein capture, immobilization, and functionalization. The binding between biotin and streptavidin or avidin is one of the strongest known interactions, characterized by rapid binding and high specificity. Labeling purified proteins with biotin is an efficient method for protein capture, immobilization, and functionalization. Biotin is a cofactor for carboxylases present in all living organisms. Proteins bind to tightly defined genomic loci and can interact with DNA at various time points for functional regulation. Alfa Chemistry researchers are dedicated to efficient in vivo and in vivo protein biotinylation services, providing professional service strategies to achieve efficient biotinylation. Efficient biotinylation service has shown great application potential in the field of protein research, and can be used as an important research tool for in vivo or in vitro biology.

Alfa Chemistry has a professional biotinylation team that can provide customized services according to customer needs. We are committed to efficient in vivo or in vitro protein biotinylation services, including but not limited to:

• High-throughput protein biotinylation

In biochemistry, a more useful protein biotag is biotin, which can be efficiently conjugated with streptavidin or avidin.

• Promiscuous protein biotinylation

High-quality biotinylated proteins are currently a major challenge, often suffering from inadequate labeling and large batch-to-batch variability. The method of biotinylation can significantly affect the results, and since each protein is unique, it is important to design a unique and efficient biotinylation strategy.

• Site-specific protein biotinylation

Protein-protein interactions (PPIs) are involved in many physiological roles, such as cell cycle division and cell signaling, and have important implications for human health and disease.

• Site-directed biotinylation

Site-directed biotinylation of antibodies is used for efficient antibody immobilization, and when biotinylated intact antibodies, biotinylation can occur at the sulfhydryl group.

• In vivo protein biotinylation

The biotinylation reaction of protein can be carried out by enzymatic biotinylation and chemical biotinylation. Enzymatic biotinylation is a biotinylation reaction facilitated by biotin protein ligase (BPL). Chemical biotinylation is usually the modification of amino groups of proteins with biotin-N-hydroxysuccinimide or similar acylating agents.

• In vitro protein biotinylation

In vitro protein biotinylation can be divided into in vitro protein biotinylation and in vitro enzymatic protein biotinylation, which can be widely used in the fields of biochemistry, cell biology and biological detection.

Our service advantages

• We have a professional team and experienced researchers to support efficient biotinylation services
• Precise biotinylation solutions and strategies
• Efficient and professional biotinylation data analysis
• Support customized services and enable efficient design and development

Figure 2. We support advanced biotinylation services to meet customer needs

Why choose us?

Alfa Chemistry specializes in biotinylation services, has a first-class research team and advanced experimental equipment, and has the ability to provide customers with high-quality biotinylation services. We can customize a reasonable biotinylation strategy according to the needs of customers, adhere to the self-developed biotinylation route, and have an advanced quality control platform to provide customers with satisfactory services.

Alfa Chemistry is committed to providing efficient and accurate in vivo or in vitro protein biotinylation services to meet customer needs. If you are interested in our services, please contact us immediately.

References:

1. Eunjoo. C. R.; et al. Promiscuous protein biotinylation by Escherichia coli biotin protein ligase. Protein Science. 2008, 29(144): 3043-3050.
2. Souvik. C.; et al. Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. Nature Protocols. 2006, 1: 2386-2398.

※ It should be noted that our service is only used for research, not for clinical use.