Cleavable Biotin Reagents

Cleavable biotin reagents are specialized tools in the fields of molecular biology, proteomics, and biochemical research. These reagents are designed to enable the reversible biotinylation of biomolecules, allowing for the temporary attachment of biotin to proteins, peptides, or nucleic acids. The cleavable nature of these reagents is crucial for applications where the recovery of unmodified biomolecules is necessary after a specific isolation or purification step.

Explore our complete catalog of cleavable biotin reagents to find the right solution for your research needs.

Product List

Key Features and Mechanism of Action

Cleavable biotin reagents are typically composed of a biotin moiety linked to a reactive group via a cleavable linker. The reactive group allows the biotinylation of specific functional groups on the target molecule, such as primary amines, thiols, or carboxyl groups. Once biotinylated, the target molecule can be isolated or detected using avidin, streptavidin, or their derivatives due to their high affinity for biotin.

The critical advantage of cleavable biotin reagents lies in the cleavable linker. This linker can be broken under specific conditions - such as exposure to reducing agents, changes in pH, or UV light - allowing for the release of the biotin and restoration of the native state of the biomolecule. This feature is particularly valuable in applications where the presence of biotin could interfere with downstream processes or when the recovery of the unmodified target is required for further analysis.

Figure 1. Design of the photo-cleavable biotin affinity tag^[1].

Types of Cleavable Linkers

• Disulfide Linkers

These are cleaved by reducing agents such as dithiothreitol (DTT) or tris(2-carboxyethyl)phosphine (TCEP), commonly used in redox-sensitive applications.

• Photo-cleavable Linkers

These linkers are broken upon exposure to UV light, providing a non-invasive method to cleave the biotin tag.

• Acid-labile Linkers

These are sensitive to acidic conditions and are cleaved at low pH, making them useful in environments where mild acidic conditions are applied.

Considerations for Choosing a Cleavable Biotin Reagent

A. Consider the specific conditions required for cleavage and ensure compatibility with the target molecule and subsequent experiments.

B. Ensure the chosen cleavable linker is specific for the desired cleavage conditions and does not interfere with other reactions.

C. Choose a cleavable biotin reagent that is stable under the experimental conditions, ensuring optimal performance.

Reference:

1. Chang T-C; et al. A Photo-cleavable Biotin Affinity Tag for the Facile Release of a Photo-crosslinked Carbohydrate-binding Protein. Bioorganic & Medicinal Chemistry. 2016, 24(6): 1216-1224.

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