Site-specific Protein Biotinylation

Protein-protein interactions (PPIs) are involved in many physiological roles, such as cell cycle division and cell signaling, and have important implications for human health and disease. Protein interactions can lead to cellular dysfunction. Biotin is a cofactor for carboxylases present in all living organisms. The binding between biotin and streptavidin or avidin is one of the strongest known interactions, characterized by rapid binding and high specificity. Labeling purified proteins with biotin is an effective method for protein capture, immobilization and functionalization. Proteins bind to tightly defined genomic loci and can interact with DNA at different time points for functional regulation. Proximity biotinylation technology based on target protein labeling can be used for simple and accurate detection and evaluation of in vivo protein interaction, and can be used for advanced protein labeling technology, providing an effective tool for proteomics and genomics research. Alfa Chemistry's researchers are committed to site-specific and efficient biotinylation services for proteins, and can design site-specific binding of special proteins, showing great application potential in protein biology research.

The Services

Alfa Chemistry can provide efficient biotinylation services, including specialized biotinylation strategies to meet a wide range of needs. Related services we can provide include but are not limited to:

• Site-specific biotin labeling
• Western blot detection
• Biotinylation solution
• Biotinylation degree detection
• Professional data analysis

In the future, our researchers will persist in innovation, cooperate with world-leading teams, pursue excellence, and meet customer needs with professional biotinylation services.

BirA enzyme and protein site-specific biotinylation

Biotin labeling is an effective tool for studying protein interactions. E. coli Enzymatic Biotinylation Biotin Ligase (BirA) is a highly specific covalently linked biotin to 15 amino acid AviTag peptides, resulting in a high degree of biotinylation. BirA enzymes can be used for efficient protein biomarkers. By targeting the BirA enzyme, the associated AviTag protein is biotinylated for detection by bioimaging. The great advance in biolabeling technology for BirA enzymes was due to the development of electron microscopy. Biotin ligases from E. coli or other species can be attached to peptide-tagged biotin for efficient biotinylation.

Application

Biotinylation technology is based on biotin ligase for high-efficiency biotinylation in vivo or in vitro. Avidin or streptavidin binds biotin reversibly and specifically for immobilization, purification and visualization of proteins.

Why choose us?

Alfa Chemistry focuses on efficient biotinylation strategies and provides efficient protein site-specific biotinylation services, showing significant advantages in the field of protein research. We have a professional team and are capable of providing professional and reliable services to customers around the world. We are equipped with a professional protein biotinylation system, support special independent research and development routes, adhere to an optimized service system, and continue to innovate to meet customer needs.

Alfa Chemistry focuses on site-specific biotinylation services and serves research institutions around the world to meet customer needs. If you are interested in our services, please contact us immediately.

References:

1. Michael. F.; et al. Site-specific biotinylation of purified proteins using BirA. Methods in Molecular Biology. 2014, 172(6): 171-184.
2. Souvik. C.; et al. Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. Nature Protocols. 2006, 1: 2386-2398.

※ It should be noted that our service is only used for research, not for clinical use.