Biotin Kit

Biotinylation is a widely used method in biochemistry and molecular biology to covalently attach biotin (a 244.31 Da vitamin, also known as vitamin B7) to proteins, peptides, nucleic acids, and other biomolecules. This process facilitates the subsequent detection, isolation, or immobilization of the labeled molecules due to biotin's strong affinity for avidin and streptavidin proteins.

A Biotinylation Kit provides all the necessary reagents and protocols for efficiently labeling target molecules with biotin. These kits are designed to be user-friendly, providing researchers with reliable and reproducible results. Typically, a biotinylation kit contains biotin derivatives that react with specific functional groups (e.g., primary amines, sulfhydryl groups) on the target molecule. The choice of biotin derivative, the buffer systems, and other reagents are optimized to preserve biological activity while achieving high labeling efficiency.

Explore our complete catalog of biotin kit products to find the right solution for your research needs.

Product List

Key Features of Our Biotinylation Kits

• High Affinity: Biotin's strong affinity for avidin and streptavidin allows for robust binding, making it suitable for applications such as ELISA, Western blotting, flow cytometry, and immunoprecipitation.
• Compatible with a variety of biomolecules, including proteins, peptides, oligonucleotides, and antibodies.
• Optimized reaction conditions maintain the biological activity of the labeled molecules.
• Available in different formats (e.g., NHS-ester, maleimide, hydrazide) to suit various experimental needs.
• Pre-weighed reagents and standardized protocols ensure reproducibility and ease of use.

Applications

• Proteomics: Identification and quantification of proteins in complex mixtures.
• Molecular Diagnostics: Biotinylated probes in assays such as ELISA, Southern blotting, and in situ hybridization.
• Drug Discovery: Screening of biotinylated drug candidates for binding interactions.
• Cell Biology: Tracking and visualization of biotin-labeled molecules in cells and tissues.
• Nanotechnology: Functionalization of nanoparticles with biotin for targeted delivery systems.

Considerations for Choosing the Right Biotinylation Kit

A. Target Molecule and Functional Groups: Consider the functional groups present in the target molecule (amines, thiols, carboxyls, etc.) and select the appropriate biotinylation chemistry.

B. Reaction Conditions: pH, temperature, and buffer composition can significantly affect labeling efficiency and specificity.

C. Downstream Applications: The intended application (e.g., in vivo vs. in vitro, diagnostic vs. research) determines the choice of kit.

D. Degree of Biotinylation: Over-labeling can affect the biological activity of the molecule, while under-labeling may result in weak signal detection.

Online Inquiry

Name:

Phone:

* Email:

* Quantity

mg g kg other

* Service & Products of Interest

Project Description:

* Verification Code

SEND