Biotinylation Detection

Proteins and physiological functions in organisms play an important role. In addition to completing their own functional tasks, proteins also need to combine with other proteins to jointly control physiological functions. For protein interactions, the most common assays are co-immunoprecipitation (co-IP), pull-down assays, cross-linking, and label transfer. Alfa Chemistry provides professional biotinylation testing services, including proximity biotinylation detection and non-immune biotinylation detection, mainly for protein interaction detection items. We focus on efficient responsive biotinylation projects and provide a wide range of biotinylation solutions and strategies to meet customers' requirements for samples, specifications and budgets. Such high-efficiency biotinylation detection is of great significance for system analysis and data processing, and opens up a new path for biological research.

Alfa Chemistry has a professional biotechnology team, providing professional biotinylation solutions and strategies, serving customers around the world, with high-quality biotinylation detection services, including but not limited to:

• Proximity biotinylation detects protein interactions in cells

A combination of proximity biotinylation detection and mass spectrometry (PDB-MS) can be used to efficiently detect protein interactions. By adding biotinylation enzymes, proteins in vivo can be covalently biotinylated, enabling the capture and recognition of "neighboring" proteins.

• Nonimmuno biotinylation to detect protein interactions

In addition to completing their own functional tasks independently, most proteins also need to interact with other proteins to ensure complete biological activity. Detecting protein interactions in cells is critical to understanding protein biology.

• Biotin detection of protein S-nitrosylation

Nitrosylation of proteins is a reversible process similar to phosphorylation. This process is independent of cyclic guanosine monophosphate (cGMP) nitric oxide modification, which is a new way to regulate protein function and is an important post-translational modification of proteins. S-nitrosylation modification widely exists in biological reactions and can regulate biological processes by improving protein activity and protein interactions.

The features of biotinylation detection platform

• Biotinylation services play an important role in immunoassays
• Efficient biotinylation detection enables efficient analysis of protein-protein interactions
• Professional platform provides services
• Supports biotinylation detection of multiple samples
• We support global customer service

Figure 2. We provide high-quality biotinylation detection services to meet customer needs

Why choose us?

In Alfa Chemistry, our scientific researchers are proficient in handling a variety of biotinylated samples, and can also provide sample preparation and support data analysis, focusing on efficient biotinylation project management. We keep pace with the times, insist on innovation and pursue excellence. We always insist on optimizing and perfecting the service system, providing a professional biotinylation platform, and focusing on efficient biotinylation services to meet the needs of customers.

The wide application

• Determination of interactions with membrane proteins and other peptides
• Screening of protein-protein interactions
• Construction of protein network system
• Screening for interactions or transient interactions

Alfa Chemistry takes advantage of competitive biotinylation services to provide customers with high-quality services. If you are interested in our services, please contact us immediately.

References:

1. Chen. L.C.; et al. Detection and analysis of plant protein S-nitrosylation modification. Chinese Bulletin of Botany. 2019, 54 (4): 497-502.
2. Anne. C.G.; et al. Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles. Current Opinion in Chemical Biology. 2019, 48: 44-54.
3. Huang. C.; et al. Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation. Biotechniques. 2010, 49(6): 881-886.

※ It should be noted that our service is only used for research, not for clinical use.