In Vivo Protein Biotinylation

Biotin is a naturally occurring metabolic enzyme factor that exhibits activity through the action of specific protein-biotin ligases. The physiological functions of organisms are mainly controlled and regulated by intracellular proteins. The biotinylation reaction of protein can be carried out by enzymatic biotinylation and chemical biotinylation. Enzymatic biotinylation is a biotinylation reaction facilitated by biotin protein ligase (BPL). Chemical biotinylation is usually the modification of amino groups of proteins with biotin-N-hydroxysuccinimide or similar acylating agents. Compared with chemical biotinylation, enzymatic biotinylation reaction has higher binding capacity and higher specificity, but the amount of protein that can be reacted is less. Alfa Chemistry aims to provide customers with efficient biotinylation services, has established a complete in vivo protein biotinylation system, and is committed to providing customers with satisfactory services. The in vivo protein biotinylation services we can provide include design of biotinylation strategy, chemical and enzymatic biotinylation, detection of biotinylation degree and professional data analysis, etc. Our researchers are constantly working to develop a wider range of biotinylation services to meet the needs of our customers.

The Services

Alfa Chemistry is committed to efficient biotinylation services, providing efficient biotinylation strategies, and making unremitting efforts to meet customer needs. We provides high-efficiency protein biotinylation in vivo, and related services include but are not limited to:

• In vivo enzymatic biotinylation of proteins

Biotin is a soluble small molecule produced by plants or prokaryotes, which can be non-covalently combined with avidin or streptavidin, making the biotin-avidin system widely used in the field of biotechnology. Avi tags are short peptide tags consisting of 15 amino acid residues that can be biotinylated by biotin ligase (BirA enzyme) in vivo or in vitro.

• In vivo chemical biotinylation of proteins

Chemical biotinylation is usually the modification of amino groups of proteins with biotin-N-hydroxysuccinimide or similar acylating agents. The chemical biotin is an effective tool for labeling proteins and biomacromolecules, reducing the requirement for specific binding and being more reactive.

What can we provide?

• Customized biotinylation solutions and strategies
• Unique production and R&D route
• Strict quality control platform
• Efficient biotin-avidin/streptavidin system for biotinylation reactions
• Precise biotinylation detection

Professional team strength and advanced technical support

• We are equipped with professional scientific research personnel and advanced equipment to provide efficient enzymatic biotinylation and chemical biotinylation services
• Professional quality control platform, strictly control the quality of biotinylation service
• Innovative service platform, advancing with the times, insisting on innovation

Figure 2. Innovative biotinylation platform and quality control system, capable of providing satisfactory biotinylation services to global customers

Alfa Chemistry has established a complete biotinylation system to provide global customers with high-quality in vivo biotinylation services, including efficient chemical biotinylation and enzymatic biotinylation. If you are interested in our services, please contact us immediately.

References:

1. Anne. C.S.; et al. In vivo enzymatic protein biotinylation. Biomolecular Engineering. 1999, 16(1-4): 119-125.
2. Brian K. K.; et al. High-throughput biotinylation of proteins. Methods in Molecular Biology. 2010, 498(46): 185-198.

※ It should be noted that our service is only used for research, not for clinical use.