Promiscuous In Vivo Biotinylation

The combination of biotin and streptavidin is the strongest known covalent biological interaction and has a wide range of applications in the field of biology. In addition to high-efficiency bioconjugation with avidin, biotin can also be combined with streptavidin and neutravidin to amplify the biotinylation reaction signal and greatly improve the detection sensitivity of proteins. The biological activity of biotinylated proteins is determined by the structure of the protein itself and the way of biotinylation. For special protein samples, a reasonable biotinylation strategy needs to be customized for efficient biotinylation reactions. The strong interaction between biotin and avidin/streptavidin makes it a powerful tool for protein biochemistry. Alfa Chemistry has a professional biotinylation team with many years of experience, and can obtain mixed biotinylated mutant BirA by checking the biotinylation of the mutant protein during expression in E. coli.

The Services

Alfa Chemistry is committed to providing efficient biotinylation services to customers around the world, providing efficient in vivo promiscuous biotinylation strategies to meet customer needs. The relevant in vivo complex biotinylation services we can provide include but are not limited to:

• Testing of promiscuously biotinylated proteins
• Professional biotinylation service
• Efficient promiscuous in vivo biotinylation strategy formulation
• Professional data analysis
• Determination of the degree and efficiency of biotinylation
• Quantitative analysis

Efficient in vivo promiscuous biotinylation with ultrahigh sensitivity

In biochemistry, biotinylation refers to the process of covalently attaching biotin to proteins or other biomolecules, which can be carried out by chemical biotinylation or enzymatic biotinylation. The biotinylation reaction is fast, efficient and has strong anti-interference ability. Biotin Ligase (Biotin Ligase) is encoded by the BirA gene, which can activate biotin to form biotinyl-5'-adenylic acid, and transfer biotin specifically to biotin receptor tagged proteins (such as AviTag fusion protein) , to biotinylate tagged proteins, and can also be used as a biotin operon blocker.

Application

• Professional biotinylation technology platform, providing professional in vivo biotinylation services, suitable for the application of various samples
• Unique specific binding to facilitate the biotinylation program, optimizing the process
• Professional biological detection, suitable for efficient protein interaction testing

Why choose us?

Alfa Chemistry has a professional biotechnology team, taking advantage of professional R&D routes, serving scientific research and technology institutions around the world, and providing high-quality biotinylation services. We support special responsive biotinylation projects, continuously optimize service processes, and continuously meet customer needs. We welcome customers from all over the world, continue to innovate, persist in development, and meet customer needs with emerging technologies.

Alfa Chemistry focuses on high-quality biotinylation solutions and strategies, and establishes a complete biotin system to meet customer needs. If you are interested in our services, please contact us immediately.

Reference:

1. Eunjoo. C. R.; et al. Promiscuous protein biotinylation by Escherichia coli biotin protein ligase. Protein Science. 2008, 29(144): 3043-3050.

※ It should be noted that our service is only used for research, not for clinical use.

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