Chemical Biotinylation with Biotin Isothiocyanate

The biotin-avidin/streptavidin system is a useful tool in immunology, histochemistry and in situ hybridization. Biotin-labeled antibodies can increase the sensitivity of heterogeneous bioassays through signal amplification, greatly expanding applications. Biotinylated biomolecules can be purified using affinity chromatography and detected by ELISA and blotting techniques. Avidin is easily modified and does not affect the affinity of biotin. At present, biotinylation reagents with reactivity to different functional groups of various biomolecules have been designed. Isothiocyanate (-NdCdS) is a bioconjugated functional group that reacts with the amine group of lysine residues and the N-terminus of proteins to form a stable thiourea moiety.

The biotinylation process is the process of combining biotin and avidin. Alfa Chemistry specializes in providing high-quality biotinylation services. Experienced scientific research experts can provide suitable solutions and strategies, and the related services that can be provided include but are not limited to:

• HABA determination
• Emission titration
• Isothiocyanate biotinylation

The wide application

• Purification of proteins and nucleic acids
• Protein interaction studies
• Immunoprecipitation
• Immunoassay
• Phage display
• Biopanning
• Drug screening and cell isolation

What can we offer?

• Professional HABA determination, targeted biotinylation, better control of biotinylation labeling process
• Committed to developing professional biotinylation systems to meet customer needs
• Enable efficient biotinylation services to support biomolecules with multiple functional groups

Over the years, Alfa Chemistry has taken advantage of competitive biotinylation services, provided all-round solutions, and insisted on meeting customers' needs with high-quality services. If you are interested in our services, please contact us immediately.

Reference:

1. Kenneth. K.W.L.; et al. Rhenium(I) polypyridine biotin isothiocyanate complexes as the first luminescent biotinylation reagents: synthesis, photophysical properties, biological labeling, cytotoxicity, and imaging studies. Inorg. Chem. 2008, 47(2): 602-611.

※ It should be noted that our service is only used for research, not for clinical use.