Biotinylated Antibody Service

Antibodies are easily combined with chemically modified activated biotin. Biotin labeling is simple, fast, mild and rarely inhibits antibody activity. Biotin-labeled antibodies are very stable and can be stored for many years. The background binding of avidin (streptavidin), which binds quickly and tightly, can avoid many disadvantages. When performing antibody labeling, it is necessary to have a good understanding of the performance of antibodies, cross-linking agents and markers. The reaction of biotin-labeled antibody is simple, mild and rarely inhibits antibody activity. Combining biotin with covalent antibody is a simple and direct labeling method. Alfa Chemistry researchers combine conjugation and labeling services with custom antibody services to optimize service processes and save costs.

The Services

Alfa Chemistry provides antibody labeling and modification services, supporting antibody modification and labeling with biotin, enzyme or fluorescein. Biotin modification and labeling is to covalently bind biotin to antibody molecules through chemical reactions, which can be widely used in the fields of protein chip analysis and immunoassay. The related services we can provide include but are not limited to:

• Biotin modified antibody service
• Biotinylated antibody service
• Biotin-labeled antibody detection
• Fluorescent antibody labeling service
• Biotin binding

Common biotin labeling reagents and their applications

• The enzyme horseradish peroxidase (HRP) is a commonly used enzyme in common experimental procedures
• HRP-labeled antibodies are widely used in ELISA kits and Western blot analysis
• Fluorescein isothiocyanate (FITC) is the most widely used fluorescein
• FITC-conjugated antibodies are commonly used in immunofluorescence and flow cytometry analysis

Service process

• Communicate with customer
• Identify solutions
• Conduct experiment
• Antiserum detection
• Antibody purification
• Antibody detection
• Product submission

Our custom service capabilities

Alfa Chemistry can customize professional solutions, optimize the preparation process, control time reasonably, and save customers' time and energy to the greatest extent. We guarantee that the batch gap between the various biotin-modified antibodies provided is small, which can meet the needs of experiments and scientific research. Our experienced researchers can couple highly sensitive markers to antigen and antibody molecules, and perform efficient qualitative measurements through the enhanced amplification effect of the markers. The biotin-modified antibody service provided by Alfa Chemistry can facilitate customers' experiments.

Precautions

• Each antibody can be labeled with 3-5 biotins. When labeling, the content of biotin and antibody needs to be strictly controlled
• Need to pay attention to the background combination
• There are certain requirements for the concentration of the sample
• The antibody modification reaction process is a reversible chemical reaction, and there is an essential consumption
• When there are too few reactants, it will affect the success rate of the experiment
• It is impossible to achieve 100% labeling efficiency

Alfa Chemistry can optimize the service process, save costs and energy, and is committed to providing customers with accurate services. If you are interested in our services, please contact us immediately.

Reference:

1. Welson. W. S.; et al. Design of a bifunctional fusion protein for ovarian cancer drug delivery: Single-chain anti-CA125 core-streptavidin fusion protein. European Journal of Pharmaceutics and Biopharmaceutics. 2007,65(3): 398-405.

※ It should be noted that our service is only used for research, not for clinical use.