Protein Biotinylation Services

Proteins are involved in important physiological activities, can participate in the synthesis of important hormones and enzymes, can maintain heredity and regulate immunity. The binding between biotin and streptavidin or avidin is one of the strongest known interactions, characterized by rapid binding and high specificity. Labeling purified proteins with biotin is an efficient method for protein capture, immobilization, and functionalization. The high affinity between biotin and avidin or streptavidin is not easy to be destroyed in a strict environment, and it can efficiently bind to proteins, reduce background binding, and make other substances easier to elute. Whether it is protein research or application, high-efficiency protein expression is usually required, followed by purification and separation, and it is easy to perform high-efficiency biotinylated protein immobilization, purification and separation. RNA-protein interaction networks have been implicated in human disease, including cancer and neurodegenerative diseases. Effective characterization of RNA-protein interactions is critical for the development of innovative RNA-centric therapeutic strategies. Currently, more and more studies combine protein biotinylation and drug delivery to achieve efficient drug immobilization and drug release. Alfa Chemistry provides professional in vivo and in vitro protein biotinylation, protein biotinylation fixation, purification, separation and detection related services to expand a wider range of application requirements.

The Services

Alfa Chemistry offers a wide range of biotinylation strategies to provide efficient protein biotinylation services. Our researchers focus on professional data analysis, support data comparison and feedback, and focus on efficient biotinylation services, including but not limited to:

• In vitro and in vivo protein biotinylation

The binding between biotin and streptavidin or avidin is one of the strongest known interactions, characterized by rapid binding and high specificity.

• Protein biotinylation immobilization

Protein immobilization can be carried out by means of adsorption immobilization and chemical bond covalent bond, which can enhance the stability in complex environments and improve the use value of proteins.

• Protein biotinylation purification

The combination of biotin and avidin is the strongest non-covalent interaction known in nature, so the biotin-avidin system can be used for efficient protein purification.

• Biotinylated protein isolation

Biotinylation is the process of attaching biotin to proteins and other biomacromolecules, which can be used for protein detection, purification or isolation.

• Protein biotinylation detection

In vivo labeling of proteins with covalently linked biotin moieties has emerged as a new promising tool for protein detection and purification.

• Protein biotinylation and targeted drug delivery

Currently, more and more studies combine protein biotinylation and drug delivery to achieve efficient drug immobilization and drug release.

Features and benefits of protein biotinylation platforms

• Optional sample adaptation test procedures
• Project response for protein biotinylation (fixation, purification, isolation and detection)
• Broad range of protein biotinylation detection strategies
• Various detection methods
• Support the processing of various samples
• Intelligent data analysis

Protein biotinylation

Biotinylation is the process of incorporating biotin groups into molecules to visualize specific substrates by incubating them with biotin-labeled probes and avidin or streptavidin. Biotinylated protein is the covalent combination product of biotin and protein and other macromolecules. Biotinylation is catalyzed by biotin ligase (BPL). Biotin is activated at the expense of ATP, and the activated biotin can react with nucleophiles on target proteins. BPL transfers biotin to biotin BPL transfers biotin to specific lysines on the biotin carboxyl carrier protein (BCCP) for efficient biotinylation.

Figure 2. Activated biotin can efficiently react with the target protein through the action of biotin ligase

Support professional system expansion and update

• Efficient protein biotinylation in less time
• Save samples and reagent consumption
• Support more reasonable strategies to achieve gradual system upgrades
• Scalable protein biotinylation platform

Alfa Chemistry provides efficient and professional protein biotinylation services. If you are interested in our services, please contact us immediately.

References:

1. Souvik. C.; et al. Strategies for site-specific protein biotinylation using in vitro, in vivo and cell-free systems: toward functional protein arrays. Nature Protocols. 2006, 1: 2386-2398.
2. Rina Y. P. L.; et al. Site-specific immobilization of biotinylated proteins for protein microarray analysis. Methods in Molecular Biology. 2012, 246: 85-100.
3. James. D. H.; et al. Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation. Analytical Biochemistry. 2002, 308(2): 345-357.

※ It should be noted that our service is only used for research, not for clinical use.