Microscopic Detection of in Vivo Protein Biotinylated Isoforms

In biochemistry, biotinylation refers to the process of covalently attaching biotin to proteins, nucleic acids or other molecules. Biotin is covalently linked to a specific target protein, and epitope tagging can be performed using the biotin-avidin/streptavidin protein interaction. Biotin is fused to a protein of interest and can be used to express biotinylated proteins in vivo in a wide range of biological systems. Currently, protein biotinylation in vivo has become a professional tool for cellular localization of proteins. In vivo labeling of proteins by covalent attachment of biotin moieties has emerged as a new promising tool for protein detection and purification. Alfa Chemistry has experienced researchers who can develop efficient in vivo biotinylated protein expression strategies, develop professional system biotinylated systems for efficient immunoelectron microscopy detection, and make it an effective protein detection and purification research tools and means.

The Services

Alfa Chemistry provides professional microscopic detection of protein biotinylation isomers in vivo, including but not limited to:

• Microscopic detection of protein biotinylated isoforms in vivo
• Design and optimization of detection strategies
• Detection of protein biotinylation degree
• Stable cell line generation, transient transfection, and in vivo biotinylation
• Protein biotinylation analysis
• Professional data analysis
• Immunofluorescence detection and analysis
• Fixation and embedding for electron microscopy
• Antibody and immunogold detection of labeled proteins
• Quantitative analysis of biotinylated proteins in vivo
• In vivo protein biotinylation is compatible with immunoelectron microscopy

Current research

Currently, in vivo biotinylation can be used for post-immunogold embedding labeling in immunoelectron microscopy experiments, which facilitates studies that demonstrate binding morphology and localization during experiments. The researchers study the isoforms of biotinylated proteins by immunoelectron microscopy in order to facilitate the precise expression of morphology and become an important tool for biological research.

Figure 2. in vivo biotinylation can be used for post-immunogold entrapment labeling in immunoelectron microscopy experiments

Our team support and advantages

Alfa Chemistry's research team is composed of experienced experts and advanced equipment, and its team composition is as follows:

• Professional biotechnology team can provide professional data analysis and establish a perfect quality control system
• Innovative and robust testing equipment and procedures
• Cooperating with the world's leading teams, focusing on technological innovation to meet customer needs

We focus on high-quality biotinylation detection strategies and provide professional biotinylation detection services. The advantages of our services include:

• Professional data analysis
• Support global customer payment
• Intelligent analysis platform, efficient and fast
• Optimize service system and process
• Project responsive biotinylation management

Alfa Chemistry provides customers with high-quality microscopic detection of protein biotinylation isomers in vivo to meet customer needs. If you are interested in our services, please contact us immediately.

References:

1. Antoine. V.; et al. Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform. Journal of Histochemistry & Cytochemistry. 2008, 56(10): 911-919.

※ It should be noted that our service is only used for research, not for clinical use.