Graphene Oxide Biotin for Targeted Therapy

Graphene oxide (GO) is a common material, which is the product of graphite powder after chemical oxidation and exfoliation. It is a two-dimensional material with a single atomic layer that can expand in the lateral dimension. Graphene oxide has a planar structure similar to graphene, and its surface contains a large number of active groups, such as hydroxyl (-OH), epoxy (-C(O)C-), carbonyl (-C=O), carboxyl (-COOH) and ester group (-COO-). Provide specific targeting ligands for the carrier, such as antibodies, vitamins, magnetic particles, genes and peptides, aiming to synthesize specific drug targeting carriers. There are van der Waals force and π-π stacking effect between graphene sheets, which can be used as an efficient carrier to bind drugs, thereby performing coupling for efficient drug delivery. Targeted drug delivery enhances drug accumulation at tumor sites to reduce exposure to normal healthy tissue. Targeted drug carrier systems based on graphene oxide have attracted much attention in recent years due to their excellent performance. Biotin-specific receptors are overexpressed on the surface of cancer cells and can be delivered as functionalized drugs targeting cancer cells.

The Services

Alfa Chemistry has a professional laboratory and a professional research team to develop a variety of biotin nanocarriers for the development of drug delivery. Our scientific research team focuses on the development and preparation of drug carriers, drug encapsulation, drug delivery and drug release. Services that can be provided on biotin-graphene oxide-based drug delivery systems include but are not limited to:

• Synthesis and preparation of graphene oxide for drug delivery
• Drug loading and release studies
• Drug release studies
• Cell viability studies for assays
• In vitro drug release studies
• Morphological changes of cancer cells in vitro
• Cytotoxicity assay
• In vitro evaluation of the hyperthermic effect

Graphene oxide material

Graphene oxide (GO), its common states are powder, sheet and solution. The oxygen-containing functional groups on the surface of graphene oxide make graphene more active and improve its performance. Graphene oxide flakes are the product of graphite powder after chemical oxidation and exfoliation. Graphene oxide is a single atomic layer that can expand to tens of microns in the lateral dimension at any time. The structure of graphene oxide spans the typical scale of chemistry and materials science, and it is regarded as an unconventional type of biosoft material. Graphene oxide can exhibit both hydrophilic and hydrophobic properties, which can be used to reduce the energy between interfaces. Graphene oxide has excellent physical, chemical, optical and electrical properties, and has a wide range of applications in optoelectronics, solar cells, flexible sensors and biology.

Characterization techniques for related materials

• Fourier Transform Infrared Spectroscopy (FT-IR)
• X-ray Diffraction Analysis (XRD)
• Transmission Electron Microscopy (TEM)
• Scanning Electron Microscope (SEM)
• Atomic Force Microscopy (AFM)
• Raman Analysis
• Study of Drug Loading and Release Effects
• Study on Drug Encapsulation Efficiency
• Cell Viability Assay
• Adenosine Triphosphate (ATP) Assay
• Lactose Dehydrogenase (LDH) Assay
• Statistical Analysis

Current research

Currently, the researchers successfully synthesized κ-carrageenan-grafted graphene oxide and biotin-coupled nanocarriers for targeting cervical cancer. Doxorubicin (DOX) is a common anticancer drug that is used to treat many types of cancer. Graphene oxide is a single atomic layer, which can expand to tens of microns in lateral dimension at any time, and can be combined with doxorubicin through π-π stacking interaction for efficient drug delivery. The material can be characterized by a variety of characterization methods, and the biotin-dominated graphene oxide carrier is considered to be an efficient delivery system for cervical cancer therapeutic drugs.

Figure 3. Biotin-dominated graphene oxide carrier is considered to be an efficient delivery system for cervical cancer therapeutic drugs

Alfa Chemistry has long been focusing on research in the field of biotin-based graphene oxide, which can be modified and improved for efficient targeted drug delivery. If you are interested in our services, please contact us immediately.

References:

1. Nicolò. M.; et al. Biotin-containing reduced graphene oxide-based nanosystem as a multieffect anticancer agent: combining hyperthermia with targeted chemotherapy. Biomacromolecules. 2015, 16(9): 2766-2775.
2. Nazanin. R.; et al. Recent trends in targeted therapy of cancer using graphene oxide-modified multifunctional nanomedicines. Journal of Drug Targeting. 2016, 25(4): 202-215.
3. Kandasamy. V.; et al. Development of biotin molecule targeted cancer cell drug delivery of doxorubicin loaded κ-carrageenan grafted graphene oxide nanocarrier. Materials Science and Engineering: C. 2019, 100: 676-687.

※ It should be noted that our service is only used for research, not for clinical use.