Metabolic Biotinylation Services

Biotin (vitamin H) is a small molecule present in all living cells and is covalently bound as a coenzyme by carboxylases and decarboxylases. Biotin ligase (BirA) facilitates biotinylation in vivo or in vitro, linking biotin to specific proteins. Biotin is an essential biomolecule in the process of biological metabolism. Biotin-labeled proteins facilitate detection, labeling, immobilization, and purification. Biotin labeling is widely used in drug targeting, gene therapy and targeting strategies. The specificity of biotin binding to proteins has a wide range of applications, including protein detection, antigen and nucleic acid analysis, drug targeting, targeted in vivo imaging, and immunotherapy. Biotin has a high affinity for avidin, a tetrameric glycoprotein secreted in egg whites that can be used for cross-linking, staining or targeted therapy. The chemical process of biotinylation often results in non-specific labeling and inactivation of the target protein. Biotinylation is performed in vitro or in vitro by the enzymatic action of a biotin ligase. Alfa Chemistry's researchers are committed to high-quality metabolic biotinylation services to meet and exceed customer needs.

The Services

Alfa Chemistry has an experienced research team that has been engaged in biotinylation-related research for a long time. The related services that can be provided include the following:

• Metabolic biotinylation of recombinant proteins

Recombinant protein is a protein obtained by applying recombinant DNA/RNA technology. Recombinant proteins have batch-to-batch consistency and better performance. The emergence of recombinant proteins has accelerated the breakthrough of protein structure and function, and cell pathways have entered the field of life science research.

• Metabolic biotinylation on cell surface

Biotin (vitamin H) is a small molecule present in all living cells and is covalently bound as a coenzyme by carboxylases and decarboxylases. Biotin ligases post-translationally covalently link the biotin moiety to specific lysine residues in the BAP sequence in these enzymes.

• Biotin ligase and metabolic biotinylation

Biotin ligase is used to catalyze the biotinylation reaction, linking biotin to specific proteins. Biotin-labeled proteins facilitate detection, labeling, immobilization, and purification. Biotin labeling is widely used in drug targeting, gene therapy and targeting strategies.

• Metabolic biotinylation of lentiviral pseudotypes

Lentiviral vector is a gene therapy vector developed on the basis of HIV-1 (human immunodeficiency virus type I). Unlike general retroviral vectors, it has the ability to infect both dividing and non-dividing cells.

• Metabolic biotinylation and purification of multiple AAV vector serotypes

Adeno-associated virus (AAV), also known as adeno-associated virus, belongs to the family Parvoviridae and the genus Reliant Virus. Adeno-associated viruses are simple, single-stranded DNA deficient viruses that require a helper virus to replicate. Adeno-associated virus can infect many kinds of cells and shows great application potential in human gene therapy.

Biotin-avidin is an efficient detection system

There is a strong interaction between the biotin-avidin system, which is one of the strongest interactions known so far. This system enables efficient modification and labeling by coupling antibodies to biotin, avidin and other biomolecules. The strong biotin-avidin interaction has high specificity and will be used to detect and/or purify proteins. The system has the advantage of multi-stage amplification of the detection signal, and the detection sensitivity is greatly improved.

Biotin and biotinylated conjugates for biological applications

Biotinylation reagents can incorporate difficult-to-detect molecules onto probes, allowing them to be recognized by labeled detection reagents or substrates with affinity capture capabilities. After the substance to be detected is labeled with biotin, the target molecule can be used to detect various biological reagents. Alfa Chemistry can provide comprehensive services related to metabolic biotinylation to meet and exceed customer needs.

In Alfa Chemistry, the researchers have years of experience to provide professional biotinylation services. Our researchers are dedicated to developing novel biotinylation systems for a wide range of applications. If you are interested in our services, please contact us immediately.

Reference:

1. Darren. N.; et al. Metabolic Biotinylation Services for Scalable Paramagnetic. Microparticle-Dependent Manipulation. MOLECULAR THERAPY. 2006, 13(4): 814-822.

※ It should be noted that our service is only used for research, not for clinical use.