Sulfhydryl-reactive Biotinylation Reagents

Sulfhydryl-reactive biotinylation reagents are specialized chemical tools used to covalently attach biotin molecules to proteins, peptides, or other biomolecules containing free sulfhydryl (-SH) groups. These reagents are pivotal in various biochemical and molecular biology applications, including protein purification, detection, and immobilization. By targeting cysteine residues, which contain reactive thiol groups, these biotinylation reagents offer a highly specific and efficient labeling strategy.

Explore our complete catalog of sulfhydryl-reactive biotinylation reagents to find the right solution for your research needs.

Product List

Examples of Sulfhydryl-Reactive Biotinylation Reagents

We offer a range of sulfhydryl-reactive biotinylation reagents tailored to meet the specific needs of your research:

• Maleimide-Biotin

A popular choice for thiol-specific biotinylation, maleimide-biotin forms stable thioether bonds with free sulfhydryl groups. This reagent is ideal for labeling proteins, peptides, and other thiol-containing biomolecules.

• Iodoacetyl-Biotin

This reagent provides an alternative sulfhydryl-targeting approach, forming covalent bonds via the alkylation of thiol groups. It is particularly useful when maleimide chemistry is unsuitable or when a different labeling strategy is required.

• Pyridyldithio-Biotin

For applications requiring reversible biotinylation, pyridyldithio-biotin offers a cleavable disulfide bond that can be selectively reduced. This feature allows for controlled release of the biotin tag under specific conditions.

Figure 1. Examples of sulfhydryl-reactive biotinylation reagents.

Usage Guidelines

For optimal results, follow these recommended steps when using sulfhydryl-reactive biotinylation reagents:

A. Ensure that your target molecule has accessible free thiol groups. If necessary, reduce disulfide bonds to generate free sulfhydryl groups using a mild reducing agent.

B. Dissolve the biotinylation reagent in an appropriate solvent, such as DMSO or DMF, to achieve the desired concentration. Avoid exposure to moisture and light, which can degrade the reagent.

C. Mix the biotinylation reagent with your target molecule under conditions that promote efficient reaction, typically at pH 6.5-7.5 and at room temperature. Adjust the molar ratio based on the degree of labeling required.

D. After the reaction, quench any unreacted reagent with a suitable quenching agent, such as cysteine or 2-mercaptoethanol. Purify the biotinylated product using desalting columns, dialysis, or affinity chromatography to remove excess reagent and byproducts.

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