Carboxyl-reactive Biotinylation Reagents

Carboxyl-reactive biotinylation reagents are a specialized class of chemical tools designed to attach biotin molecules to carboxyl-containing biomolecules covalently. These reagents are widely used in biochemistry, molecular biology, and biotechnology for labeling proteins, peptides, antibodies, and other biomolecules that have free carboxyl groups (-COOH). The biotinylation of carboxyl groups is crucial for various applications, including affinity purification, immunoassays, protein-protein interaction studies, and the development of biotin-streptavidin-based detection systems.

Explore our complete catalog of carboxyl-reactive biotinylation reagents to find the right solution for your research needs.

Product List

Mechanism of Action

Carboxyl-reactive biotinylation reagents typically consist of a biotin moiety linked to a reactive group capable of forming covalent bonds with carboxyl groups. The most common reactive group used for this purpose is carbodiimide, which facilitates the formation of an amide bond between the carboxyl group of the target molecule and an amine group introduced by the reagent. Another commonly used reactive group is hydrazide, which reacts with carboxyl groups in the presence of carbodiimide or directly with activated carboxyl groups. These reactions ensure stable and specific biotin attachment, preserving the functional integrity of the biomolecule.

Figure 1. The carboxyl amino acid side chains of Bovine Serum Albumin model protein were activated by EDC and Sulfo-NHS, and then biotinylated by Biotinyl Cystamine^[1].

Product Selection

When selecting a carboxyl-reactive biotinylation reagent, it is essential to consider factors such as the type of biomolecule to be labeled, the desired degree of biotinylation, and the intended application. Our product range includes various carboxyl-reactive reagents with different spacer lengths, solubilities, and reactivity profiles to suit diverse experimental needs.

Key Advantages of Carboxyl-reactive Biotinylation Reagents

• High Specificity - Carboxyl-reactive biotinylation reagents offer high specificity, targeting only carboxyl groups on the biomolecule, thus minimizing non-specific labeling.
• Versatility - These reagents are compatible with a wide range of biomolecules, including proteins, peptides, nucleic acids, and small molecules.
• Stability - The covalent bond formed during the biotinylation process ensures stable attachment of biotin, even under varying experimental conditions.
• Ease of Use - Carboxyl-reactive biotinylation reagents are user-friendly, with straightforward protocols that can be easily integrated into existing workflows.

Reference:

1. Müller A; et al. Covalently Modified Carboxyl Side Chains on Cell Surface Leads to a Novel Method toward Topology Analysis of Transmembrane Proteins. Scientific Reports. 2019, 9: 15729.

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