Nucleic Acid Delivery Based on Avidin-biotin System

Nucleic acids, including plasmid DNA, siRNA, miRNA, aptamers, and oligonucleotides, are widely used as therapeutic agents for a variety of diseases. In recent years, nucleic acid therapy has made breakthroughs. A system suitable for nucleic acid drug delivery needs to consider that it should have excellent biocompatibility to avoid immunogenic reactions. At present, liposomes, peptide nanocomposites and nanoparticles are currently used for efficient nucleic acid delivery systems, which can also be modified and improved to meet the delivery requirements of nucleic acids. The negative charge and poor stability of nucleic acid are important factors affecting the function of nucleic acid. Based on the excellent pharmacokinetics of avidin, after drug delivery in vivo, the drug-loaded system will be internalized, enabling targeted delivery at specific sites. The avidin-biotin interaction has attracted a lot of attention, and the system has the advantages of being safe and easy to operate. The avidin-biotin system can be easily modified functionally, so that it can target a variety of ligands, and carry out targeted drug delivery and treatment of diseases in a multifunctional manner. In the future, more and more researches will focus on drug-targeted systems for various diseases, which will meet the application requirements with unique advantages.

The Services

Alfa Chemistry has a team with rich experience and deep background knowledge, focusing on the research of avidin-biotin system and expanding its application in the field of biomolecular delivery. Its related services include but not limited to:

• Nucleic acid delivery test based on avidin-biotin system
• Synthesis and preparation of materials suitable for avidin-biotin system
• Characterization of materials suitable for avidin-biotin system
• Cytotoxicity assay
• Cellular uptake study

Challenges of nucleic acid delivery systems

1. Nucleic acid is easily degraded

2. The surface is negatively charged and it is difficult to cross the cell membrane

Delivery platform for nucleic acid therapeutics

• Antisense oligonucleotides (ASOs)
• N-acetylgalactosamine ligand-modified short interfering RNA conjugate (GalNAc−siRNA)
• Lipid Nanoparticles (LNPs)
• Adeno-associated viral vector (AAV)

Quality control of nucleic acid delivery systems

The effect of nucleic acid drug delivery in vivo depends on the performance of the following carrier materials:

• Composition and properties
• Particle size distribution
• Structure
• Appearance
• Surface charge
• Hydrophilic properties
• Drug loading
• Types of surface ligands
• Density of surface ligands

Understanding the properties of nucleic acid delivery system materials is of great significance for the design, development and quality control of nucleic acid delivery systems.

Future development

Nucleic acid drug delivery has gone through a long process of development, and technological breakthroughs play an important role in the development of efficient nucleic acid delivery, which is also the focus of future research. Through the strategy of targeted selective performance, it can be endowed with special surface properties to promote efficient nucleic acid delivery, and it is expected to be used in clinical research.

Alfa Chemistry researchers are committed to developing a variety of new multifunctional materials suitable for efficient nucleic acid delivery in the avidin-biotin system. If you are interested in our services, please contact us immediately.

References:

1. Ravi. S. S.; et al. Development of Streptavidin-Based Nanocomplex for siRNA Delivery. Mol. Pharmaceutics. 2013, 10: 4534-4545.
2. Péter. H.; et al. Functionalized liposomes loaded with siRNAs targeting ion channels in effector memory T cells as a potential therapy for autoimmunity. Biomaterials. 2013. 34(38): 10249-10257.

※ It should be noted that our service is only used for research, not for clinical use.