Branched Biotin Reagents

Branched biotin reagents are specialized chemical tools used extensively in molecular biology and biochemistry. These reagents are designed to introduce multiple biotin moieties into biomolecules, enabling enhanced detection, purification, and immobilization of target molecules. Their branched structure provides a higher density of biotin tags, increasing the binding capacity and sensitivity in various applications.

Product List

Alfa Chemistry's catalog offers a range of branched biotin reagents tailored to different experimental requirements, including varying linker lengths, spacer chemistries, and functional group compatibilities. Each product is rigorously tested to ensure high purity and performance in your biotinylation applications.

Key Features of Branched Biotin Reagents

• High Biotin Density

The branched architecture allows for multiple biotin groups to be attached to a single molecule, significantly increasing the biotinylation efficiency and enhancing the binding strength with streptavidin or avidin.

• Enhanced Signal Amplification

Due to the high density of biotin tags, these reagents are ideal for applications requiring signal amplification, such as Western blotting, ELISA, and immunohistochemistry.

• Versatility

Branched biotin reagents can be used with a wide range of biomolecules, including proteins, peptides, nucleic acids, and small molecules, making them versatile tools for diverse experimental needs.

• Stable and Reliable

The reagents are designed to be stable under a variety of conditions, ensuring consistent and reproducible results across different experiments.

Figure 1. Some types of branched biotin reagents.

Choosing the Right Branched Biotin Reagent

The selection of the appropriate branched biotinylation reagent depends on the specific application and target molecule. Factors to consider include:

• Target molecule - The nature of the target molecule (e.g., protein, antibody, nucleic acid) will determine the appropriate functional group for labeling.
• Reaction conditions - The pH, temperature, and buffer conditions should be compatible with the chosen reagent.
• Application - The specific application (e.g., immunoprecipitation, ELISA) will influence the desired properties of the reagent, such as avidity, sensitivity, and cleavability.

Advantages Over Linear Biotin Reagents

A. The branched structure provides a higher biotin-to-molecule ratio compared to linear biotinylation reagents, leading to stronger interactions with avidin or streptavidin and improved assay sensitivity.

B. The multivalency of branched biotin reagents enhances the binding kinetics, offering faster and more stable interactions with biotin-binding proteins.

C. The branched configuration minimizes steric hindrance, allowing for better accessibility of biotin tags to their binding partners, which is particularly beneficial in complex biological environments.

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