Avidin-biotin Complexes for Affinity Chromatography

The specific interaction between biomolecules has high binding force, such as antigen-antibody interaction, enzyme-substrate interaction, hormone-receptor interaction and biotin-avidin interaction, etc. The separation principle of affinity chromatography is based on the immobilization of affinity-based biomolecules on an insoluble matrix, and the use of high-efficiency affinity energy can purify another molecule. The ligands immobilized on the matrix can be used to purify antibodies, enzymes, hormones and other biomolecules through interaction. The biotin-avidin system has the strongest known non-covalent interaction force and can be used for affinity chromatography of biomolecules. Biotin columns are based on biotin for the separation of avidin, streptavidin, anti-biotin antibodies and avidin conjugates for further separation and characterization.

The Services

The researchers of Alfa Chemistry are committed to expanding the application of the biotin-avidin system in the field of affinity chromatography. The related services that can be provided include but are not limited to:

• Design and expansion of biotin-avidin system
• Biotin-avidin system for affinity chromatography
• Biotin-avidin system for purification and separation of avidin
• Purification and separation of streptavidin by biotin-avidin system
• Biotin-avidin system for purification and separation of anti-biotin antibodies
• Biotin-avidin system for purification and separation of avidin conjugates

Biotin-avidin system for affinity chromatography

Choosing the right affinity adsorbent is a key factor for efficient affinity chromatography. The biotin-avidin system can be used for affinity chromatography, with biotin as the matrix ligand, which can be used for efficient purification of avidin, streptavidin, anti-biotin antibodies and avidin conjugates. The choice of affinity chromatography matrix needs to take into account the following factors:

• Stable physical and chemical stability
• High affinity for ligand
• The structure of the matrix has a porous network structure

About affinity chromatography

Affinity chromatography is used to separate and purify specific interactions between ligands and target molecules. Alfa Chemistry provides customers with professional affinity chromatography-related services and insists on meeting customer needs.

What can we provide?

• Professional biotin-avidin system
• Efficient affinity chromatography separation and purification services
• Processing and analysis of various samples
• Data analysis and comparison

Biotin-avidin complex

Avidin-biotin complexes have a wide range of applications in bioanalysis and are commonly used in the professional field of biology. Based on the principle of non-covalent interaction, the biotin-avidin complex can be used in the fields of affinity chromatography, medical diagnosis and medical positioning. The biotin-avidin complex utilizes robust interactions and can be used for separation and purification by affinity chromatography. In the future, a wider range of applications will be expanded to meet a wide range of needs.

Alfa Chemistry has an experienced team that can expand the application of the biotin-avidin system in the field of affinity chromatography, which can be widely used in the separation and purification of multiple biomolecules. If you are interested in our services, please contact us immediately.

Reference:

1. Meir. W.; et al. The Avidin-biotin complex in bioanalytical applications. ANALYTICAL BIOCHEMISTRY. 1988, 171: 1-32.

※ It should be noted that our service is only used for research, not for clinical use.