In Vivo Biotinylation of Antigens

Antigen refers to a substance that can cause an immune response and can be combined with specific antibodies in the environment for potential biological therapy. Antigens are exogenous proteins or fragments that enter the host through infection. Bacteria or viruses contain antigens on or within them that can be used to develop highly effective vaccines. Antigens have relatively high molecular weight, usually proteins, polysaccharides, nucleic acids, polypeptides, lipids, nucleic acids or other biomolecules. Antibodies and other affinity reagents are valuable resources for studying protein function and distribution, expanding the field of research to validate and select antibodies for human proteins. Antibodies against foreign antigens are a key component of the immune system, enabling efficient antigen clearance and protection against subsequent infections. Antibody responses can lead to autoimmune diseases, malignant tumors or enhanced infection, so providing high-quality antigens is of great significance for the development of specific protein-targeting antibodies. The production of affinity reagents for a large number of human proteins depends on the ability to express the target protein as a high-quality antigen. More and more ways are used to express biotinylated antigens, using high-efficiency expression vectors, which are usually biotinylated by metabolic reactions in E. coli.

The Services

Alfa Chemistry focuses on optimizing the service system and provides a wide range of biotinylation strategies and solutions to meet customer needs. The related services we provide about biotinylated antigens in vivo include but not limited to:

• Antigen biotinylation in vivo
• Sample incubation, transfection and screening
• Purification of biotinylated molecules
• quantitative analysis
• Determination of biotinylation efficiency
• In vivo site-specific biotinylation

About antigens and the immune system

An antigen is any substance that can cause an immune response, and general biomolecules can be used as antigens. Foreign molecules can directly activate B cells through B cell receptors, bind to major tissues to form complexes, activate on T cells, and trigger an immune response. The main characteristic of an antigen is its immunogenicity, which induces both humoral and cellular immune responses in the body. Antigens are usually proteins, polysaccharides, lipids, polypeptides, carbohydrates, nucleic acids or biomolecules. Some small substances (haptens) can also be chemically coupled to larger molecular weight carrier proteins, and can also generate immune responses. Some small molecule drugs, such as monosaccharides, amino acids and phospholipids, can also act as haptens and cause immune responses.

Why choose us?

• Self-developed biotinylation route
• Broad range of biotinylation solutions and strategies
• Optimized service process
• Professional Data Analysis
• Professional biotinylation project management

Alfa Chemistry provides efficient in vivo antigen biotinylation services and focuses on data analysis to meet customer needs. If you are interested in our services, please contact us immediately.

References:

1. Jascha-N. R.; et al. In vivo protein biotinylation for identification of organ-specific antigens accessible from the vasculature. Nature Methods. 2005, 2: 291-298.
2. Susanne. G.; et al. In vivo biotinylation of antigens in E. coli. Heterologous Gene Expression in E.coli. 2017, 04: 337-344.

※ It should be noted that our service is only used for research, not for clinical use.