Detection of Biotinylated Proteins in Polyacrylamide Gels

The high-affinity binding of biotin and avidin has been used as a molecular tool in a wide range of biotechnology, biodiagnostics and medical treatments. Avidin is a tetrameric glycoprotein that tetramerizes biotinylated proteins. Biotinylation can not only increase the binding affinity of avidin, but also increase the multivalency and improve the functionality of the protein. In addition to the in vitro biotinylation of the target protein, in vivo biotinylation applied to the overexpression of recombinant proteins in E. coli is valuable in various fields such as proteome research. Currently, biotinylated proteins after electrophoresis can be detected directly in gels using avidin-fluorescein conjugates and fluorescence image analyzers. The method can also be used to specifically detect the biotinylated protein on the cell surface of Escherichia coli, and the detection method has high sensitivity, strong specificity and simple operation. Alfa Chemistry's research team is composed of experienced researchers who can choose appropriate strategies according to the characteristics of customers' samples, support efficient data analysis, and provide customers with efficient protein biotinylation detection services.

The Services

Alfa Chemistry's researchers are committed to developing efficient protein biotinylation detection methods, focusing on providing a wide range of biotinylation strategies, including but not limited to:

• Preparation of fluorescein-labeled antibody
• Detection of biotinylated proteins in gels using avidin-fluorescein conjugates
• Detection of chemically biotinylated proteins in the gel
• Detection of enzymatically biotinylated proteins in gels
• Professional data analysis

Current research

Currently, the researchers have used avidin-fluorescein conjugates and fluorescence image analyzers for efficient detection of biotinylated proteins. This method has a wide range of applications and can be applied to chemically biotinylated protein samples as well as in vivo enzymatically biotinylated protein samples. The method has low cost, simple operation, high sensitivity and high-quality specificity, and it is expected to be used in a wider range of research fields.

Professional digital testing platform

Alfa Chemistry has a world-class digital detection platform, equipped with experienced researchers and complete equipment, capable of meeting the biotinylation detection needs of various customers. The first-class digital detection platform, professional data analysis, and special detection procedures can effectively solve various problems encountered in biotinylation detection.

• Intelligent data analysis, supporting data comparison, so as to facilitate the next experiment
• Variety of biotinylation programs, you can choose the program suitable for the sample for detection
• A wide range of detection schemes to adapt to the testing of various samples

Alfa Chemistry provides professional detection services for biotinylated proteins in polyacrylamide gels, meeting customer needs with accurate test results. If you are interested in our services, please contact us immediately.

References:

1. Michihiro. N.; et al. Detection of biotinylated proteins in polyacrylamide gels using an avidin-fluorescein conjugate. Analytical Biochemistry. 2002, 304(2): 231-235.

※ It should be noted that our service is only used for research, not for clinical use.