Biotin Ligase and Metabolic Biotinylation

Biotin ligase is used to catalyze the biotinylation reaction, linking biotin to specific proteins. Biotin-labeled proteins facilitate detection, labeling, immobilization and purification. Biotin labeling is widely used in drug targeting, gene therapy and targeting strategies. Biotin labeling of drugs, proteins or viruses is performed chemically in vitro. Usually biotinylation will cause other couplings, which will lead to the inactivation of the biological function of the substrate or cross-linking, which may affect the binding characteristics and cause the loss of affinity. Biotin ligase can be secreted from cells and can perform extracellular protein biotinylation. Biotin ligases can also be immobilized in the endoplasmic reticulum (ER), where substrate proteins are biotinylated as they pass through the ER. The use of biotin ligase can effectively carry out biotinylation, which is of great significance for the production of biotinylated recombinant proteins.

The Services

Alfa Chemistry has a professional technical team and has been engaged in biotinylation services for a long time. The related services it can provide include but are not limited to:

• Gene assembly of fusion proteins
• Design of a biotin ligase system retained in the endoplasmic reticulum
• Solution design and development
• Biotinylated purification and expression
• Characterization of purified biotinylated proteins

About biotin ligase

Biotin ligase (Biotin Ligase, BirA, EC 6.3.4.15) can activate biotin to form biotinyl-5'-adenylate, and transfer biotin specifically to biotin receptor protein (such as AviTag fusion protein), to biotinylate the protein. Currently, researchers are working on developing important systems for metabolic biotinylation based on biotin ligases. The newly developed in vivo metabolic biotinylation method is a general method for the biotin-avidin system.

Technical support

We have a professional technical team, timely technical guidance, assist customers to solve experimental problems, and are willing to establish a long-term cooperative relationship with you.

Production and scientific research

Alfa Chemistry has many years of experience in the field of biotinylation, and has built a number of biological research platforms and research centers, providing a basic guarantee for the innovation of biotechnology. At the same time, we also have professional technology and ability to insist on providing high-quality biotinylation services. In addition to professional team strength, we also have an efficient production center, an advanced instrument center (equipped with PCR instruments, biological safety cabinets, nucleic acid and protein analyzers, high-pressure cell disruptors, FPLC and other series of high-end precision instruments and equipment) and a research and development team.

Application

Suitable for biotinylation of biotin receptor proteins.

Service introduction

The biotin ligase is compiled and expressed by the BirA gene in Escherichia coli, which can catalyze the biotinylation reaction and covalently link biotin to specific proteins. A specific protein refers to a tagged protein containing AviTag, through the modification of biotin, directional biotinylation can be achieved. Biotin ligase is expressed by a specific gene, has the characteristics of high-speed purification, high efficiency, and high stability, and is suitable for efficient in vitro biotinylation of recombinant proteins.

In Alfa Chemistry, researchers can support efficient biotinylation services. If you are interested in our services, please contact us immediately.

Reference:

1. Bhaswati. B.; et al. Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum. Biomolecular Engineering. 2007, 24(3): 283-291.

※ It should be noted that our service is only used for research, not for clinical use.