Carbonyl-reactive Biotinylation Reagents

Carbonyl-reactive biotinylation reagents are essential tools in biochemical and molecular biology research, offering a specific and efficient method for labeling proteins, peptides, and other biomolecules containing carbonyl groups. These reagents are designed to form stable covalent bonds with carbonyl moieties, such as aldehydes and ketones, which are often present in oxidized glycoproteins, carbohydrates, and other biomolecules. The resulting biotinylated molecules can be detected, isolated, or immobilized using avidin or streptavidin-based systems, enabling a wide range of downstream applications.

Explore our complete catalog of carbonyl-reactive biotinylation reagents to find the right solution for your research needs.

Product List

Examples of Carbonyl-Reactive Biotinylation Reagents

These reagents typically contain a biotin molecule attached to a reactive group that specifically interacts with carbonyl groups.

• Hydrazide-based Reagents

These reagents react with carbonyl groups to form stable hydrazone bonds, making them suitable for labeling oxidized sugars and glycoproteins.

• Aminooxy-based Reagents

Aminooxy reagents form oxime bonds with carbonyl groups, providing an alternative labeling strategy with different reaction kinetics and conditions.

• Alkoxyamine-based Reagents

Alkoxyamine reagents are highly reactive toward carbonyl groups, offering rapid and efficient biotinylation under mild conditions.

Figure 1. Structure of the carbonyl-reactive linker biocytin hydrazide^[1].

Key Features of Carbonyl-reactive Biotinylation Reagents

• Specificity - Carbonyl-reactive biotinylation reagents exhibit high specificity for carbonyl groups, minimizing non-specific labeling and preserving the native structure and function of the target biomolecules.
• Versatility - These reagents are compatible with various biomolecules, including proteins, peptides, carbohydrates, and small molecules, making them highly versatile for different biotinylation applications.
• Stability - The covalent bonds formed between the carbonyl groups and the biotinylation reagents are stable under physiological conditions, ensuring that the biotinylated molecules maintain their integrity throughout the experimental process.
• Sensitivity - Biotinylated molecules can be detected with high sensitivity using avidin or streptavidin-conjugated detection systems, such as horseradish peroxidase (HRP), alkaline phosphatase (AP), or fluorescent labels.

Figure 2. Biotinylation of protein reactive carbonyls with biotin hydrazide^[2].

Considerations for Using Carbonyl-Reactive Biotinylation Reagents

A. Ensure that the target molecule contains accessible carbonyl groups for efficient labeling.

B. Optimize reaction conditions, such as pH, temperature, and reagent concentration, to achieve optimal labeling efficiency.

C. Consider the potential for non-specific labeling of other functional groups.

D. Ensure that the formed biotin-conjugate is stable under the desired experimental conditions.

References:

1. Fahrer J; et al. Quantitative Analysis of the Binding Affinity of Poly(ADP-ribose) to Specific Binding Proteins as a Function of Chain Length. Nucleic Acids Research. 2007, 35(21): e143.
2. Codreanu SG; et al. Global Analysis of Protein Damage by the Lipid Electrophile 4-Hydroxy-2-nonenal. Mol Cell Proteomics. 2009, 8(4): 670-680.

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