Biotinylation Service

Biotin is a water-soluble small molecule that can bind to avidin or streptavidin with high affinity and specificity. Biotinylation is the process of covalently linking biotin to nucleic acid, protein or nucleic acid molecule by chemical or enzymatic means, forming a biotin-avidin/streptavidin system. Biotin is an ideal choice for labeling proteins and biomacromolecules, mainly because of its small molecular weight, strong anti-interference ability, and high specific binding. The covalent bond present in the biotin-avidin/streptavidin system is known to be one of the strongest forces. The biotinylation reaction is fast, efficient, and insensitive to environmental factors such as temperature, pH, and proteases. BirA is a biotin ligase that catalyzes the covalent attachment of biotin to specific lysine residues of BAP fused to recombinant proteins. In addition to high-efficiency bioconjugation with avidin, biotin can also be combined with streptavidin and neutravidin to amplify the biotinylation reaction signal and greatly improve the detection sensitivity of proteins. Alfa Chemistry is committed to efficient biotinylation services, including enzymatic biotinylation, chemical biotinylation, biotinylation detection, biotinylation expression and in vivo biotinylation. Our researchers optimize the service system and provide a wide range of biotinylation strategies to meet customer requirements for samples, specifications and budgets. This kind of biotinylation system is of great significance to the analysis and processing of data, and provides a new way for biological research.

The Services

Alfa Chemistry has a strict quality control platform and a complete service system, focusing on providing efficient biotinylation strategies to meet customer needs. Related services we can provide include but are not limited to:

• Enzymatic biotinylation

Enzymatic biotinylation is one way of biotinylation. The attachment of biotin to proteins is a specific post-translational modification catalyzed by biotin ligases, the most common of which is the protein BirA enzyme from Escherichia coli.

• Chemical biotinylation

Biotinylation is the process of attaching biotin to proteins and other macromolecules. Biotinylation reagents can be used to target specific functional groups or residues, including primary amines, thiols, carboxyl groups, and carbohydrates.

• Biotinylation detection

In addition to completing its own functional tasks, proteins also need to combine with other proteins to jointly control physiological functions. For protein interactions, the most common detection methods are co-immunoprecipitation (co-IP), pull-down assays, cross-linking, and label transfer.

• Biotinylated expression, purification and characterization

The biotinylation reaction is fast, efficient and interference insensitive (unaffected by heat, pH and proteolysis). Efficient expression and characterization of biotinylation has important implications for understanding biology.

• In vivo biotinylation service

The combination of biotin and streptavidin is the strongest known covalent biological interaction and has a wide range of applications in the field of biology.

About biotin and avidin/streptavidin

Biotin is a water-soluble small molecule of the vitamin B family, also known as vitamin H, vitamin B7, and coenzyme R. Biotin can bind to avidin or streptavidin with high affinity and specificity. The biotin-avidin/streptavidin system is to form a covalent interaction between biotin and avidin/streptavidin through chemical or biological means. The system has the advantages of high sensitivity, high stability, high binding force, good specific binding and wide application range. The biotin-avidin/streptavidin system can be widely used in biological testing, genetic testing, affinity analysis and localization testing and other fields.

The advantages of biotinylation

The biotinylation system can be widely used in the fields of biological diagnosis, affinity chromatography, localization observation and gene probe, and has the following significant advantages:

• High sensitivity: Biotin can combine with proteins and random biological macromolecules to form covalently bound biotinylated macromolecules and markers. The biotinylation system has the function of amplifying the signal of the tinylation reaction, which can greatly improve the sensitivity of detection.
• Excellent specificity: The binding force between avidin and biotin is very strong and has a high degree of specificity. The binding force of the biotinylation system is not affected by the concentration of the reagent, thereby reducing the non-specific effect of the reagent.
• High stability: In the biotin system, the stability of the product is high, which can improve the accuracy of the determination.
• Universality: The biotin system has a wide range of applications and is multi-functional, which can meet the detection and experimental applications of various instruments.

Figure 2. Efficient biotinylation system and perfect service system to meet customer needs

Professional biotinylation platform to meet specifications

At Alfa Chemistry, our experienced researchers focus on high-quality biotinylation strategies and insist on expanding the biotinylation database to meet a wide range of applications. We have established a complete quality control system to meet the requirements of industry norms. We have the ability to provide customers with accurate and efficient biotinylation services, and make unremitting efforts to meet the needs of customers.

Alfa Chemistry has a complete quality assurance system, strictly controls the quality of service, and insists on providing efficient in vivo biotinylation detection services for global customers. If you are interested in our services, pleasecontact us immediately.

References:

1. Li. H.R.; et al. Thiol-cleavable biotin for chemical and enzymatic biotinylation and its application to mitochondrial turboID proteomics. J. Am. Soc. Mass Spectrom. 2021, 32(9): 2358-2365.
2. Kenneth. K.W.L.; et al. Rhenium(I) polypyridine biotin isothiocyanate complexes as the first luminescent biotinylation reagents: synthesis, photophysical properties, biological labeling, cytotoxicity, and imaging studies. Inorg. Chem. 2008, 47( 2): 602-611.
3. Huang. C.; et al. Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation. Biotechniques. 2010, 49(6): 881-886.

※ It should be noted that our service is only used for research, not for clinical use.