Protein and Peptide Delivery Based on Avidin-biotin System

Therapeutic peptides and proteins are of great significance in the treatment of diseases and have attracted considerable interest. During the delivery of macromolecules such as peptides and proteins, the biggest problem is encapsulation. Proteins and peptides require specific activities to enter the transport machinery inside the cell. With the rapid development of nanomedicine, it is of great significance to study the properties of nanocarriers. Peptides and proteins are easily deformed, resulting in a reduction in activity, so it is necessary to avoid the reduction in activity caused by deformation during the delivery of proteins and peptides. Due to the simplicity of biotinylation of proteins or peptides, avidin can be used to modify highly efficient delivery vehicles. Biotin has a small structure and low steric hindrance, which can modify proteins or polypeptides and avoid restrictions caused by conformational changes. Avidin modification not only provides an efficient carrier for the delivery of proteins and peptides, but also greatly promotes the internalization of cells. The mechanism of avidin can combine with four biotin sites to achieve targeted delivery.

The Services

Alfa Chemistry has a team with rich experience and deep background knowledge, focusing on the research of avidin-biotin system and expanding its application in the field of biomolecular delivery. The protein delivery services based on the avidin-biotin system we can provide include but are not limited to:

• Protein delivery tests based on the avidin-biotin system
• Synthesis and preparation of materials for avidin-biotin system suitable for protein delivery
• Characterization of materials suitable for the avidin-biotin system for protein delivery
• Peptide delivery test based on avidin-biotin system
• Synthesis and preparation of materials for avidin-biotin system suitable for peptide delivery
• Characterization of materials suitable for the avidin-biotin system for peptide delivery
• Cytotoxicity test
• Cellular uptake studies
• Detection of drug loading
• Drug release control

Quality control of protein and peptide delivery systems

Proteins and peptides are easily affected by environmental factors, causing changes in the internal conformation of the molecule, resulting in loss or loss of properties and functions. Since biotinylation of protein molecules requires minimal modification, avidin modification provides an efficient vehicle for the delivery of proteins and peptides. Understanding the properties of proteins and carriers (particle aggregation, size, and surface properties) is important for developing avidin-biotin-based protein delivery systems. Selection of appropriate drug delivery vehicles opens up a wide range of targeted modification strategies for effective quality control.

Why choose us?

Alfa Chemistry has the ability to develop high-quality, multifunctional nanomaterials, and strives to expand the application in the field of biomolecular delivery. We have an experienced team, cooperate with the world's top teams, and adhere to a wide range of strategies to meet the needs of customers.

Our team support

• Antibody production technicians with professional technical training
• Standardized barrier facilities, in line with industry standards

The researchers of Alfa Chemistry strive to expand multifunctional materials suitable for the drug delivery field of avidin-biotin system. If you are interested in our services, please contact us immediately.

References:

1. Akshay. J.; et al. The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis. J Control Release. 2017, 10(245): 27-40.
2. Scott. E.; et al. Martin non-natural cell surface receptors: synthetic peptides capped with N-cholesterylglycine efficiently deliver proteins into mammalian cells. Bioconjugate Chem. 2003, 14: 67-74.

※ It should be noted that our service is only used for research, not for clinical use.