In Vivo Biotin Recombinant Antibodies

Yeast produces biotinylated recombinant antibodies in vivo. Researchers have now developed a new method to generate in vivo biotinylated recombinant antibodies secreted by yeast. In vivo biotinylation supports efficient biotinylation for accurate testing. Yeast is a single-celled fungal organism that has played an important role in the field of biogenetics in recent years. Based on the expansion system of the yeast gene codon, it can exploratoryly use site-directed co-translational modification as a research method for synthetic epigenetics. Through the functionalized introduction group, co-translation can be realized to modify and maintain the life activity of yeast. In-depth research on yeast can provide efficient ideas and perspectives in the long modification process. Yeast is an important tool for genetic research. Knocking out genes in it can realize the processing of genetic modification.

The Services

Utilizing the important role of yeast in genetic research, the research of biotinylated recombinant protein antibody in vivo can be realized. Alfa Chemistry researchers have been engaged in in vivo biotinylation services for a long time, and can perform professional yeast gene knockout, making it an important tool in genetic research. The related services we can provide include but are not limited to:

• In vivo biotinylation using yeast
• Yeast gene knockout service
• Development and optimization of biotinylated antibodies
• Assay development and optimization
• Antibody detection
• Homologous recombination in yeast

Quality control standards

• Professional colony PCR identification
• Target site sequencing identification
• Precise yeast targeting

What are recombinant antibodies?

Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination and are produced by immunizing animals with an antigen to elicit an immune response. Polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, whereas monoclonal antibodies are derived from a single B cell clone and are specific for only a single epitope. Recombinant antibodies are monoclonal antibodies. The antibody gene is cloned into an expression vector, which is transfected into a suitable host cell line for antibody expression. Mammalian, bacterial, yeast, and insect cell lines are all suitable for recombinant antibody production.

Preparation method of biotinylated antibody

• Active biotin derivatives bind to functional groups on protein surfaces
• Site-specific biotinylation of recombinant antibodies

Recombinant antibody: no animal origin

Production of recombinant antibodies avoids the use of animals, and recombinant antibodies are purified from TCS transfected host cell lines

Service introduction

Based on typical antibody production, Alfa Chemistry can optimize the antibody production process and develop efficient gene knockout methods, which can be widely used in genetic gene editing.

Service process

• Consulting and communication
• Provide professional solutions
• Sign the contract and send samples
• Expand the experiment
• Project report

Figure 2. We can provide professional recombinant antibody production services and can perform efficient gene knockout

Alfa Chemistry's researchers focus on the development and preparation of highly efficient recombinant antibodies, which can be widely used in the field of biological detection. If you are interested in our services, please contact us immediately.

Reference:

1. Nathalie. S.; et al. Method for generation of in vivo biotinylated recombinant antibodies by yeast mating. Journal of Immunological Methods. 2006, 317(1-2): 132-143.

※ It should be noted that our service is only used for research, not for clinical use.