Synthesis of Luminescent Biotinylation Reagents

Biotin modification of biomolecules (biotinylation) is widely used in the purification, recognition and immobilization of biomolecules due to its fast, strong and specific avidin-biotin interaction. The biotin-avidin system is a powerful tool for immunology, histology and in situ hybridization. The biotinylated system usually retains the biological activity and characteristics of common biomolecules, and a variety of biomolecules have been found to be highly reactive to biotinylated systems. Fluorescent dyes are one of them. Avidin is a glycoprotein that can be extracted from egg white. Avidin is easily combined with biotin, and can also be modified with fluorescent reagents without affecting the binding ability of biotin. The fluorophore can be combined with a highly efficient biotin-avidin system and can be used in an efficient biological detection system. Luminescent biotinylation reagents are one of the key directions of current research, and the luminescent properties of biotinylation systems will be used in a wide range of biological assays. Biotinylated biomolecules are studied by luminescence spectroscopy.

The Services

Alfa Chemistry researchers can provide efficient biotinylation services, and can design and synthesize efficient biotin-avidin systems. The biotin-avidin system with luminescent properties can be widely used in the field of biological diagnosis. The services we can provide include but are not limited to:

• Design and development of luminescent biotin-avidin system
• Cytotoxicity assay
• Detection of electron absorption and emission properties of luminescent biotin-avidin system
• Biological detection
• Solution design
• Interaction detection of fluorescein-biotin-avidin system
• Luminescent optical assay
• Determination of the degree of biotinylation

Current research

The researchers developed a rhenium (I) biotin isothiocyanate complex as the first class of luminescent biotinylation reagents. The biotin-avidin system has a high degree of modification performance, and can be covalently bonded to a variety of functional groups to achieve functional applications. The isothiocyanate complex provides highly efficient luminescent properties for the biotin-avidin system, that is, the degree of biotinylation can be measured by means of fluorescence detection. This method of modifying the biotin-avidin system has high-efficiency tracking significance for biomacromolecules and substrates, and is of great significance for the development of biomedicine.

Figure 2. The luminescent group and biotin system can be combined efficiently to be widely used in the field of biological detection and diagnosis

Functional design and development of biotinylation system

Based on the fact that the biotinylation system can efficiently combine with various functional groups, other functional patterns can be functionally modified without changing the basic biotinylation system. Combining the group addition strategy and the structure simplification strategy, the functionalized biotinylation system is transformed by introducing external group modification, or group replacement of functional groups, branched chain derivation, etc., to provide customers with diversification, the system with rich functions and novel structure can widely meet the application requirements of customers.

Alfa Chemistry can provide the synthesis and design of biotin-avidin system, which can covalently link the luminescent group to the biotinylation system, so as to be applicable to a wide range of biological testing fields. If you are interested in our services, please contact us immediately.

References:

1. Siu-Kit. L.; et al. Design of Luminescent Biotinylation Reagents Derived from Cyclometalated Iridium(III) and Rhodium(III) Bis(pyridylbenzaldehyde) Complexes. Inorg. Chem. 2010, 49: 4984-4995
2. Kenneth. K. L.; et al. Rhenium(I) polypyridine biotin isothiocyanate complexes as the first luminescent biotinylation reagents: synthesis, photophysical properties, biological labeling, cytotoxicity, and imaging studies. Inorg. Chem. 2008, 47: 602-611.

※ It should be noted that our service is only used for research, not for clinical use.

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