Biotin Labeling Service

The biotin-streptavidin system is a system that can amplify biological signals, so it can be widely used in biological immunology. Biotin can have high affinity and binding force with chicken albumin, avidin, fungal protein and streptavidin, forming a typical bio-avidin system. The biotin-avidin system has the function of multi-stage signal amplification, and can be combined with various immunological techniques such as luciferin, enzyme or isotope, and has become one of the important tools in modern biological research. The interaction between biotin and avidin can be used in protein purification, detection, immobilization, drug targeting and protein structure analysis. The combination of biotin and other biomolecules can be regarded as biotin labeling and modification, showing the advantages of being more stable, more accurate, and more convenient, and has huge potential applications in the field of more precise detection.

The Services

The combination of biotin and avidin/streptavidin has a high degree of specificity, and the interaction between biotin and avidin/streptavidin is very strong, which is the highest covalent interaction known so far, which makes this system a useful tool in biological diagnosis. The researchers of Alfa Chemistry have been engaged in the research of biotin for a long time. They can design and measure customized schemes according to the characteristics of samples, and insist on providing complete biotin labeling and modification services. The related services we can provide are as follows:

• Biotinylated protein service

Biotin and streptavidin have a high affinity, and can be organically combined with immunolabeling techniques such as fluorescein, enzyme, and isotope to further improve the specificity and sensitivity of immunity.

• Biotinylated antibody service

Antibodies are readily conjugated to chemically modified activated biotin. Biotin labeling is simple, rapid, gentle and rarely inhibits antibody activity. Biotinylated antibodies are very stable and can be stored for many years.

• Biotin modification service

Biotin-labeled modification has the advantages of high affinity and multi-stage amplification, and can be combined with various immunological techniques such as fluorescein, enzyme or isotope, and has become one of the important tools in modern biological research.

Top partners, professional technical support

• We maintain long-term partnerships with the world's leading biotechnology companies, master new technologies, and provide customers with accurate and efficient services
• Experienced experts who have worked in related fields for many years can reasonably design and improve
• Leading technical team, focusing on the development and expansion of new technologies

Biotin labeling using advanced technology

Biotin is easy to combine with protein and nucleic acid biomolecules to form biotin derivatives. Based on the high affinity of biotin and avidin, multi-stage amplification can be performed, which can greatly improve the sensitivity of the detection method. The reaction of biotinylation and modification is simple, fast and gentle.

• Biotin labeling occurs on protein residues, and the labeling is specific
• Does not affect the activity of the modified substrate

Biotin labeling and modification by chemical or biological means

Our researchers have developed a variety of optimized systems that can be adapted to chemical methods for biotin labeling and modification. It usually allows the protein to bind to multiple biotin molecules, which has the following advantages

• High sensitivity
• Easy to mark
• Fast

High batch-to-batch consistency

We have a strict quality control system to ensure the consistency between batches of products. Each series of products will be compared and tested internally through a variety of experimental methods, and only products within the allowable range can be used. For the labeling and modification of biotin, the results of experiments at the cellular level are highly consistent.

Alfa Chemistry can provide biotin labeling and modification services. If you are interested in our services, please contact us immediately.

Reference:

1. Welson. W. S.; et al. Design of a bifunctional fusion protein for ovarian cancer drug delivery: Single-chain anti-CA125 core-streptavidin fusion protein. European Journal of Pharmaceutics and Biopharmaceutics. 2007,65(3): 398-405.

※ It should be noted that our service is only used for research, not for clinical use.