In Vivo Chemical Biotinylation of Proteins

Biotinylation is the process of attaching biotin to proteins and other macromolecules for detection and purification of proteins or antibodies. Biotinylation of proteins can also be done chemically in addition to enzymatically. Chemical biotinylation is usually the modification of amino groups of proteins with biotin-N-hydroxysuccinimide or similar acylating agents. Enzymatic biotinylation is usually highly specific and the number of proteins that can be modified is small. The chemical biotinylation reduces the requirement for specific binding and is also more reactive. The chemical biotin is an effective tool for labeling proteins and biological macromolecules, and has the advantages of small molecular weight and strong anti-interference ability. The chemical biotinylation we can provide includes amino biotinylation, sulfhydryl biotinylation, hydroxyl biotinylation and carboxylic acid biotinylation. In the future, our researchers will still be committed to the research of biotinylation for a long time to meet a wider range of applications. Alfa Chemistry's researchers focus on high-quality in vivo chemical biotinylation services, and are committed to providing satisfactory and suitable biotinylation strategies for customers around the world to meet their needs.

The Services

Alfa Chemistry focuses on efficient in vivo protein biotinylation services, provides a wide range of biotinylation strategies and solutions, and constantly improves the service system. We provide professional chemical biotinylation services, including but not limited to:

• Efficient in vivo biotinylation solutions and strategies
• Efficient in vivo protein biotinylation
• Professional data analysis
• Biotinylation degree detection and analysis
• Protein biotinylation detection
• Quantitative analysis

In the future, our researchers will continue to develop biotinylation strategies and expand the extensive database to be applicable to a wider range of protein samples, so as to perform efficient protein biotinylation reactions.

In vivo chemical biotinylation

Alfa Chemistry has a professional biotechnology team that supports a variety of in vivo protein biotinylation services and provides efficient biotinylation strategies. Our experienced scientific staff can carry out efficient data management and analysis, and can develop suitable solutions for different samples. We can support multiple chemical biotinylation:

• Amine biotinylation
• Thiol biotinylation
• Carbonyl biotinylation
• Carboxylic acid biotinylation

What can we provide?

Alfa Chemistry has a first-class biotinylation team, equipped with an advanced quality control platform, supports self-developed biotinylation routes, insists on optimizing service processes, and supports global biotinylation orders to meet customer needs.

• Customized body chemical biotinylation service
• Support unique independent research and development routes
• Professional biotinylation platform
• Strict quality control system
• Suitable and perfect biotinylation solution
• Efficient biotin-avidin/streptavidin system
• Support global customer payments
• Precise biotinylation detection

Alfa Chemistry has a professional biotinylation detection platform that provides professional protein biotinylation in vivo and supports efficient chemical biotinylation. If you are interested in our services, please contact us immediately.

Reference:

1. Brian K. K.; et al. High-throughput biotinylation of proteins. Methods in Molecular Biology. 2010, 498(46): 185-198.

※ It should be noted that our service is only used for research, not for clinical use.