Antibody Biotinylation Services

Biotin can form a stable interaction with avidin or streptavidin, and can be widely used in the fields of biotechnology and medical diagnosis. Biotin can be combined with biomolecules to form an efficient biotin system. The antibody can be labeled with 3-5 biotins. At present, immunoconjugates and multispecific antibodies have become efficient research tools. Biotin can be attached to the fixed position of the antibody to form biotinylated antibody. Biotinylated antibodies are currently commonly used reagents for molecular diagnosis, while recombinant antibodies are monoclonal antibodies that can be functionally designed. Functionalized single-strand biotinylated fragments can be prepared by a simple preparation method for efficient and sensitive immunoassay applications. Antibody biotinylation becomes progressively more precise, forming precise covalent conjugates that are less susceptible to temperature, pH, organic solvents, and denaturants.

The Services

Alfa Chemistry can provide professional biotinylation services, antibody biotinylation is an important service, including but not limited to:

• Antibody enzymatic biotinylation

Antibodies are widely used in typical biological diagnostics. After biotin or avidin is combined with antibody molecules or markers, it will not affect the affinity of the former, nor will it change the characteristics of the antibody. Antibody biotinylation becomes progressively more precise, forming precise covalent conjugates that are less susceptible to temperature, pH, organic solvents, and denaturants.

• In vivo biotin recombinant antibodies

In vivo biotinylation supports efficient biotinylation for accurate testing. Based on the yeast gene codon expansion system, site-directed co-translational modification can be exploited exploratoryly as a research method for synthetic epigenetics.

• Biotin single chain antibody fragments

Antibodies are composed of immunoglobulin domains, each of which contains an intradomain disulfide bond that plays an important role in stabilizing the protein structure. Functionalized single-strand biotinylated fragments can be prepared by simple preparation methods for efficient and sensitive immunoassay applications.

• Antibody site-specific biotinylation

Biotin can be attached to the fixed position of the antibody to form a biotinylated antibody, which can be used in the fields of biomarking and diagnosis. Biotinylated antibodies are currently commonly used reagents for molecular diagnosis, while recombinant antibodies are monoclonal antibodies that can be functionally designed.

The main method of biotin-avidin labeling technology

• Bridging avidin-biotin labeling method (BAB): The bridging avidin-biotin labeling method can be divided into direct method and indirect method. Directly use free avidin or streptavidin as a bridging agent to combine biotinylated antibody complexes with labeled biotin, so that efficient detection can be performed. In the indirect method, after the antigen is combined with the specific antibody, the biotinylated second antibody is combined with the antigen-antibody complex, which improves the sensitivity of the reaction.
• Avidin-biotin-peroxidase complex method (ABC): Mix avidin/streptavidin and enzyme-labeled biotin in a certain ratio to form a streptavidin-biotin-peroxidase complex.
• Labeled avidin-biotin method (LAB): This method includes direct method and indirect method. The direct method is to directly link the labeled avidin or streptavidin to the biotinylated antibody in the immune complex for enzymatic color reaction. The indirect method uses a biotinylated secondary antibody to bind to the antigen, and its detection sensitivity is higher than that of the direct method.
• Labeled streptavidin-biotin method (LSAB): The labeled streptavidin-biotin method is to detect cells and tissues by linking a biotin-labeled secondary antibody to streptavidin conjugated with horseradish peroxidase (HPR) or alkaline phosphatase (AKP).
• Photobiotin method (Photobiotin): Photosensitive biotin is a chemically synthesized biotin derivative. The molecule contains a light-activated azide-nitrophenyl group. Under short-term irradiation of visible light, it can react with the basic group of nucleic acid to produce a biological Primer-labeled nucleic acid probes.

About Us

Alfa Chemistry has many years of experience, with years of technology accumulation, continuous process optimization and cost control, can provide customers with high-quality biotinylation services. Our researchers have been engaged in related industries for many years and can provide customers with suitable reagents by using the appropriate biotinylation reagent selection tool.

Alfa Chemistry provides professional biotinylation services to serve biological customers around the world to meet their needs. If you are interested in our services, please contact us immediately.

Reference:

1. Welson. W. S.; et al. Design of a bifunctional fusion protein for ovarian cancer drug delivery: Single-chain anti-CA125 core-streptavidin fusion protein. European Journal of Pharmaceutics and Biopharmaceutics. 2007,65(3): 398-405.

※ It should be noted that our service is only used for research, not for clinical use.