Proximity Biotinylation Detects Protein Interactions in Cells

Services

The combination of proximity biotinylation detection and mass spectrometry (PDB-MS) can be used to efficiently detect protein interactions. These powerful technical principles are based on enzyme and protein fusions that allow efficient expression in biological environments. By adding biotinylation enzymes, proteins in vivo can be covalently biotinylated, thus enabling the capture and recognition of "neighboring" proteins. At present, researchers have reported a simple and fast yeast detection method that can be used to efficiently screen interactions between proteins. Using the protein arginine methyltransferase Rmt3 and the RNA exosomal subunits Rrp6 and Dis3, protein-protein interactions can be examined and screened by TurboID, a recently described version of the BirA biotin ligase. Alfa Chemistry's researchers have long been committed to high-quality biotinylation services, mastered advanced protein labeling techniques, and can perform efficient detection of adjacent biological proteins. Our researchers can conduct systematic analysis and quickly develop effective detection methods, opening up new channels for proteomics research.

Alfa Chemistry has developed a high-efficiency biotinylation detection platform, which can provide professional protein interaction detection, and professionally provide related detection services, including but not limited to:

Proximate protein biotinylation labeling
Proximity protein biotinylation for efficient detection of protein interactions
Protein extraction and western blotting
Affinity capture of biotinylated proteins

Biological significance

Neighboring proteins can be efficiently biotinylated, thus enabling efficient detection and identification of protein-protein interactions. Complementation is often performed in a variety of ways to successfully detect protein-protein interactions. Rapid and systematic analysis can be carried out by using the proximity biotinylation detection platform, which provides a new channel for proteomics research.

Our team support and advantages

Our advanced team with experienced scientific experts can support proximity biotinylation services for efficient detection of protein interactions
We cooperate with the world's leading research teams, carry out scientific research exchanges, and master advanced technologies
We insist on innovation, develop new detection platforms, and focus on efficient biotinylation services to meet customer needs
Global service support, efficient biotinylation service, perfect service system

Figure 2. We support high-quality biotinylation services, providing a wide range of solutions and strategies to meet customer needs

Broad application of proximity biotinylation

Identify interactions with membrane proteins and other peptides
Enrichment of transient and/or low affinity interactions not easily captured by affinity purification methods
Understanding the organization of membraneless organelles and other subcellular structures that are not easily isolated or purified
Screening of protein-protein interactions
Construction of protein network system
Screening for interactions or transient interactions

Alfa Chemistry has developed a professional biotinylation platform, can detect protein interactions through adjacent biotinylation reactions, providing a new and efficient detection method. If you are interested in our services, please contact us immediately.

References:

Marta. F.S.; et al. Protein−protein interaction detection in vitro and in cells by proximity biotinylation. J. Am. Chem. Soc. 2008, 130(29): 9251-9253.
Anne. C.G.; et al. Getting to know the neighborhood: using proximity-dependent biotinylation to characterize protein complexes and map organelles. Current Opinion in Chemical Biology. 2019, 48: 44-54.

※ It should be noted that our service is only used for research, not for clinical use.

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