Enzymatic Biotinylation of Fab Fragments

There are noncovalent interactions between proteins and small organic molecules with high affinity, and the biotin-avidin/streptavidin system is used in biotechnological and diagnostic applications. The researchers successfully fused recombinant anti-estradiol Fab fragments to a specific site-specific enzyme for biotinylation. The Fab peptide fusion protein is secreted into the periplasm of E. coli, purified, and biotinylated in vitro using biotin ligase, biotin, and ATP. In biodiagnostics, streptavidin-coated tubes, microtiter wells or spherical particles are used as versatile surfaces for capturing biotinylated antibodies in various forms of immune responses. Site-specific biotinylated Fab fragments were tested in streptavidin-coated microtiter wells and showed specific binding. Enzymatic biological Fab fragments with uniform antigen affinity have great potential for immunoassays. Alfa Chemistry offers a wide range of biotinylation strategies to meet client time and budget for biotinylation services. We have experienced, highly skilled and dedicated scientists to provide the most suitable method for each biotinylation project.

Alfa Chemistry focuses on high-quality biotinylation services, providing a wide range of solutions and strategies to meet different customer requirements for specifications, standards, time and budget. Our team is made up of an experienced team of scientists who specialize in providing the right method for each biotinylation project. Over the years, we have won the industry's appreciation for our high-quality biotinylation services. Our services include but are not limited to:

• Efficient enzymatic biotinylation of Fab fragments
• Construction of biotin receptor peptide
• Determination of biotinylated Fab Fragments
• Purification of biotinylated Fab fragments
• Design of Fab-biotin receptor peptide fusion protein
• Affinity calculation

Why choose us?

Alfa Chemistry has a professional biotechnology team, scientific researchers with many years of experience in the industry, equipped with advanced professional equipment, and is committed to providing the world's most advanced biotinylation services and support. Our researchers specialize in a variety of biotinylated samples, including enzymatic biotinylation of Spodoptera frugiperda cells, enzymatic biotinylation of Fab fragments, and in vivo biotinylation of peptide complexes. Our experts can also perform sample preparation, sample purification, analysis of initial data and experimental data, and are dedicated to providing biotinylation-related support to meet the specific needs of our customers.

Figure 2. We have the ability to provide customers with high-quality biotinylation services to continuously meet customer needs

What can we offer？

Over the years, Alfa Chemistry has strictly adhered to relevant quality assurance and industry norms, and has provided high-quality services with efficient quality. We are committed to raising the industry standard and can provide customized biotinylation services according to the customer's service.

• Data Analysis of our support experiments
• Provide high-quality biotinylation services for efficient Fab fragment biotinylation
• With years of experience, we can provide professional solutions and strategies
• Design of specialized biotin-avidin/streptavidin systems

Alfa Chemistry is committed to high-quality biotinylation reagents, providing high-quality services at cost-effective prices. If you are interested in our services, please contact us immediately.

Reference:

1. Petri. S.; et al. In vitro enzymatic biotinylation of recombinant Fab fragments through a peptide acceptor tail. Bioconjugate Chem. 1998, 9(6): 725-735.

※ It should be noted that our service is only used for research, not for clinical use.