Metabolic Biotinylation of Lentiviral Pseudotypes

Lentivirus vector is a gene therapy vector developed on the basis of HIV-1 (human immunodeficiency virus type I). Different from general retroviral vectors, it has the ability to infect both dividing cells and non-dividing cells. Proteins on the surface of lentiviral vectors have important effects on the biology of the vector, facilitating infection and conferring resistance to complement inactivation. Biotin ligase (BirA) facilitates biotinylation in vivo or in vitro. Cell-derived lentiviral vectors have proteins on their surface that can be biotin-modified and labeled. Now, researchers have demonstrated that biotinylated lentiviral vectors have a high affinity for streptavidin paramagnetic particles, allowing the development of new methods for purification and concentration. Human cells covalently couple exogenous biotin to specific receptors. Researchers can design metabolic biotin labeling systems for viral vectors that can be used for efficient biotinylated expression.

The Services

Alfa Chemistry can provide professional lentivirus metabolic biotinylation services, including but not limited to:

• Extended Processing Samples
• Capture and enrichment of lentiviruses
• biotin-dependent capture
• Lentiviral Vector Production
• Staining and visualization of lentivirus/particle complexes
• Solution development and design
• Lentiviral packaging service

Lentiviral vectors and metabolic biotinylation

In recent years, research on lentiviral vectors has gained a deep understanding and has also developed rapidly. Human immunodeficiency virus (HIV), simian immunodeficiency virus (SIV), equine infectious anemia (EIA), feline immunodeficiency virus (FIV) are lentiviruses. Lentiviral vectors effectively integrate foreign genes into host chromosomes, and can effectively infect neuronal cells, liver cells, cardiomyocytes, tumor cells and endothelial cells. Lentivirus is also widely used in the research of expressing RNA.

About lentivirus

Lentivirus is a reliable tool developed on the basis of HIV-1 (Human Immunodeficiency Virus Type I), which can be applied to efficient gene therapy. A notable feature of diseases caused by lentiviruses is a long incubation period. Lentiviral vectors can effectively integrate foreign genes into the host chromosomes to achieve persistent expression, which has significant advantages in gene therapy. In recent years, retroviral and lentiviral vectors have become important tools in a variety of experiments, often used in animal models, gene therapy and genetic diagnosis.

Significance of lentiviral vector development

• It can greatly improve the transduction efficiency of the target gene, and provides an effective way for RNAi, cDNA and other cloning and reporter gene research to achieve rapid gene expression
• Convenient for the screening of professional and specific cell lines
• Provide high-quality gene fragments for the study of living cells

Vector construction and technical support

Lentiviral vectors have the characteristics of wide host range, low immunogenicity, large gene capacity, and long-term expression. It can effectively infect cultured tumor cells, liver cells, cardiomyocytes, neurons, endothelial cells, stem cells and other types of cells. Alfa Chemistry has a professional technical team that can construct highly efficient lentiviral vectors, which is of great significance for improving gene transfection efficiency and for further gene therapy and analysis.

Advantages of lentiviral vectors

• Long expression time: the gene can be expressed for a long time without loss
• High safety: no pathogenicity
• Low immunogenicity: used in a wide range of animal experiments

In Alfa Chemistry, the researchers have years of experience to provide professional biotinylation services. Our researchers are dedicated to developing novel biotinylation systems for a wide range of applications. If you are interested in our services, please contact us immediately.

Reference:

1. Darren. N.; et al. Metabolic Biotinylation of Lentiviral Pseudotypes for Scalable Paramagnetic. Microparticle-Dependent Manipulation. MOLECULAR THERAPY. 2006, 13(4): 814-822.

※ It should be noted that our service is only used for research, not for clinical use.