Biotinylated Peptides, Ligands, Antibodies and Proteins

Biotinylation is a widely employed technique in biochemistry and molecular biology for the modification of peptides, ligands, antibodies, and proteins. By attaching biotin molecules to these biomolecules, researchers can harness the strong affinity between biotin and avidin/streptavidin for various applications, including purification, detection, and immobilization. The versatility of biotinylation allows for the creation of tailored biotin-conjugated biomolecules that maintain their biological activity while offering enhanced functionality.

Product List

Alfa Chemistry's range of biotinylated peptides, ligands, antibodies, and proteins is designed to meet the highest standards of quality and performance. Explore our catalog to find the ideal biotinylated biomolecule for your research needs, or contact us for custom biotinylation services tailored to your specific requirements.

Various Product Categories

• Biotinylated Peptides

Peptides are often biotinylated at specific residues to enable their capture, detection, or immobilization in various assays. The biotin tag can be introduced at the N-terminus, C-terminus, or at lysine residues, depending on the desired application.

Biotinylated peptides are crucial in applications such as ELISA, Western blotting, and peptide-based affinity purification. Our biotinylated peptides are synthesized with high precision, ensuring consistent labeling and maintaining the biological activity of the peptide.

Figure 1. Biotinylated peptides (blue) are used to modify streptavidin-coated Octet biosensor tips (yellow-green), which are then dipped into antibody samples (green)^[1].

• Biotinylated Ligands

Ligands, including small molecules and other binding partners, can be biotinylated to facilitate their interaction with target proteins or receptors. Biotinylated ligands are often used in receptor-ligand binding studies, drug screening assays, and affinity purification of protein complexes. The biotin tag does not interfere with the ligand's ability to bind to its target, making these biotinylated ligands ideal for sensitive and specific applications.

• Biotinylated Antibodies

Antibodies are frequently biotinylated for use in immunoassays, including ELISA, flow cytometry, and immunoprecipitation. The biotinylation process is carefully controlled to ensure that the antibody retains its antigen-binding capability while providing multiple biotin sites for robust interaction with avidin or streptavidin.

Figure 2. Biotinylated antibodies can be bound to the solid support via streptavidin^[2].

• Biotinylated Proteins

Proteins can be biotinylated to enable their use in various detection and purification protocols. Biotinylated proteins are valuable tools in protein-protein interaction studies, pull-down assays, and as standards in various analytical techniques. The biotinylation of proteins is performed under mild conditions to preserve their structural integrity and functional activity, ensuring reliable results in downstream applications.

Key Features and Benefits

• High Specificity and Affinity - Biotin's strong affinity for avidin and streptavidin allows for highly specific interactions, minimizing background noise in assays.
• Versatility - Biotinylated biomolecules can be used in a wide range of applications, including detection, purification, and immobilization.
• Retention of Biological Activity - Our biotinylation process is optimized to preserve the biological function of peptides, ligands, antibodies, and proteins, ensuring that they perform effectively in your experiments.
• Custom Labeling Options - We offer custom biotinylation services to meet specific research needs, allowing you to choose the site and degree of biotinylation that best suits your application.

Applications

A. Biotinylated antibodies and peptides are essential for the capture and detection of target antigens in Enzyme-Linked Immunosorbent Assay (ELISA).

B. Biotinylated proteins and antibodies are used for the detection of specific proteins on blots, offering enhanced sensitivity and specificity.

C. Biotinylated ligands and proteins are employed in affinity purification techniques to isolate and study protein complexes or specific biomolecules.

D. Biotinylated antibodies allow for the specific detection and quantification of cell surface markers in flow cytometry applications.

References:

1. Shaw AE; et al. Avidity of Polyclonal Antibodies to Foot-and-Mouth Disease Virus in Bovine Serum Measured Using Bio-Layer Interferometry. Viruses. 2022, 14(4): 714.
2. Koivunen M; et al. Principles of Immunochemical Techniques Used in Clinical Laboratories. Laboratory Medicine. 2006, 37(8): 490-497.

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