Site-directed Biotinylation

Biotinylation of proteins, nucleic acids, lipids and carbohydrates is one of the most fundamental tools in modern cell biology and biotechnology. Due to the scarcity of native biotinylated proteins, biotin can bind to biopolymers and organic ligands for specific artifacts. Biotinylation is an important means for efficient affinity labeling, purification and detection of macromolecular materials. Biotin functions as a carboxyl carrier and is present in all organisms from microorganisms to mammals. In E. coli, biotin carboxyl carrier protein (BCCP) is the only protein that can be biotinylated, and BCCP is biotinylated by ATP forming an amide bond. Site-directed biotinylation of antibodies is used for efficient antibody immobilization, and when biotinylated intact antibodies, biotinylation can occur at the sulfhydryl group. Alfa Chemistry provides professional site-directed biotinylation services, mainly for the site-directed biotinylation of antibodies. Alfa Chemistry can provide professional antibody biotinylation strategies, which can achieve high-throughput and efficient biotinylation, and show great application potential in the field of protein biology.

The Services

Alfa Chemistry adheres to customer-centricity, can provide customized biotinylation services according to customer needs, and insists on meeting customer needs. We have a first-class research team who have been engaged in biotinylation services for a long time and focus on efficient biotinylation strategies. The related services we can provide include but are not limited to:

• Construction of fusion protein
• Solid phase immunoassay
• Antibody purification and isolation
• Site biotinylated protein construction

Antibody-directed biotinylation

Antibody, a typical Y-shaped protein, exists in pathogens such as bacteria and viruses, and exists in extracellular fluids such as plasma in vertebrates, and on the cell membrane surface of B cells. Antibodies can recognize specific foreign substances, thereby causing an immune response in the organism. Site-directed biotinylation of antibodies at the hinge region was developed to immobilize antigen in a directed manner by biotin-streptavidin. When reduced biotin is used to biotinylate intact antibodies, biotinylation occurs preferentially at the sulfhydryl group. Alfa Chemistry researchers have the ability to provide customers with high-quality antibody site-specific biotinylation services, can design appropriate and efficient biotinylation strategies, and insist on providing customers with efficient services.

Our service advantages

• Top team, support first-class biotinylation service
• Efficient and professional biotinylation data analysis
• Precision biotinylation solutions and strategies
• Continuously optimized service system
• Professional quality monitoring platform
• Support global customer service

What can we provide?

Alfa Chemistry has a professional biotechnology team and insists on meeting customers' needs with high-quality biotinylation services. We have a first-class team, equipped with an advanced quality control platform, can strictly control the quality of services, and insist on providing high-quality services. We can provide professional data management and data analysis, and can design and develop suitable solutions for different samples to meet customer needs. Our scientific researchers support the self-developed biotinylation strategy and route, insist on optimizing the service process, and support global customers to submit orders.

Alfa Chemistry provides customers with high-quality site-directed biotinylation services to meet their needs. If you are interested in our services, please contact us immediately.

Reference:

1. Il-Hoon. C.; et al. Site-directed biotinylation of antibodies for controlled immobilization on solid surfaces. Analytical Biochemistry. 2007, 365(1): 14-23.

※ It should be noted that our service is only used for research, not for clinical use.