High-throughput Protein Biotinylation

In biochemistry, one of the more useful biotags for proteins is biotin, which can be efficiently combined with streptavidin or avidin. The above methods of labeling and modifying proteins can be used in various biological experiments, such as affinity selection for phage display, pulling down interacting proteins from cell lysates or displaying proteins on arrays. Alfa Chemistry researchers are dedicated to site-specific and efficient biotinylation of proteins. Using an efficient biotinylation strategy, high-throughput and high-content biotinylation can be achieved. The above efficient biotinylation strategies can be used for different sources and different types of protein samples to achieve efficient purification and immobilization of proteins. We are committed to high-efficiency biotinylation strategies, which have shown great application potential in potential high-throughput proteomics, and can also be used as important tools for biological research in vivo or in vitro, providing key advantages.

The Services

Alfa Chemistry has a professional biotinylation team, which can provide efficient biotinylation strategies to support high-throughput biotinylation services, aiming to meet the needs of a wide range of adaptations. The related services we can provide include but are not limited to:

• Construction of recombinant plasmid-encoded protein
• Preparation of carrier DNA
• Construction of recombinant plasmids
• Eliminate free biotin
• Protein biotinylation verification

Methods for protein biotinylation

• In vivo chemical biotinylation of proteins
• In vivo enzymatic biotinylation of proteins
• In vitro chemical biotinylation of proteins
• In vitro enzymatic biotinylation of proteins

Significance of high-throughput biotinylation of proteins

Biotinylation at high dosage and content plays an important role in the research field of proteomics, and it can also be used as an efficient in vivo and in vitro tool. The high-throughput protein biotinylation strategy can be used in a wider range of protein applications, suitable for a variety of protein samples, and can achieve professional protein purification and immobilization.

Protein biotinylation detection method

• Enzyme-linked immunosorbent assay: It is a rapid method to verify the biotinylation of a protein of interest.
• Kit method: Use p-nitrophenyl phosphate to detect the amount of streptavidin retained into the kit, so the kit can be used to determine the amount of biotin in the protein molecule
• Electrophoresis method: The sample can be efficiently combined with streptavidin-coated magnetic beads, and the amount of non-biotinylated protein in the sample can be tracked through sodium dodecyl sulfate-polyacrylamide gel.

What can we provide?

• We support the expansion route of independent research and development
• Broad range of biotinylation solutions and strategies
• Professional biotinylation system
• Professional data analysis
• Optimized service process
• Efficient detection of protein biotinylation
• Construction of carrier

Alfa Chemistry focuses on high-throughput protein biotinylation services, providing efficient biotinylation strategies for multifunctional applications. If you are interested in our services, please contact us immediately.

References:

1. Brian K. K.; et al. High-throughput biotinylation of proteins. Methods in Molecular Biology. 2010, 498(46): 185-198.
2. Wilchek. M.; et al. Essentials of biorecognition: the (strept)avidin-biotin system as a model for protein—protein and protein-ligand interaction. Immunol. Lett. 2006, 103, 27-32.

※ It should be noted that our service is only used for research, not for clinical use.