In Vivo Biotinylated Expression System

Biotin, an essential micronutrient, is a small organic molecule that acts as a cofactor for biotin-dependent metabolic enzymes. Biotin can bind avidin or streptavidin with a very high affinity to form a biotin-avidin/streptavidin system. The high affinity between biotin and avidin or streptavidin has been widely used in biochemistry and molecular biology, usually for efficient protein detection or capture. Proteins can be covalently labeled with biotin chemically or enzymatically, which is one of the efficient protein labeling methods. The expressed biotin constructs can be fully biotinylated by co-expression with a plasmid overproducing E. coli biotin ligase. The degree of biotinylation in vivo can be promoted by adding ATP. Co-expression of biotin ligase and receptor protein can greatly facilitate the development of purification procedures. Escherichia coli biotin ligase (BirA), a bifunctional protein that acts as a transcriptional repressor to regulate biotin biosynthesis genes, covalently attaches biotin to the side chain of lysine. Alfa Chemistry provides an efficient biotinylation expression system for highly specific and quantitative in vivo biotinylation of fusion proteins to facilitate quantitative biotinylation of proteins, which can be used for purification or to facilitate interaction analysis.

The Services

Alfa Chemistry focuses on high-quality biotinylation research, which provides efficient biotinylation solutions and strategies, and we also supports biotinylation services for various samples. The services we can provide include but are not limited to:

• Design of high-efficiency expression system for biotinylation in vivo
• Detection of in vivo biotinylated expression system
• Expression of fusion protein
• Determination of protein biotinylation levels
• Quantitative analysis
• Determination of biotinylation efficiency
• Gene expression

Why choose us?

Alfa Chemistry has a professional R&D team, focusing on the analysis and experimentation of biotinylated samples, and providing efficient biotinylation solutions to global customers to meet biotinylation-related requirements. With perfect service system and sound quality control platform, we insist on providing customers with satisfactory services.

Our team strength and support

• A professional team with many years of experience in the field of biotinylation, providing customers with accurate biotinylation services and support
• Support professional technical support, providing a wide range of biotinylation solutions and strategies
• Continuously optimize the service system and process, and insist on meeting the needs of customers with efficient services

What can we provide?

• Expansion route of independent research and development
• Broad range of biotinylation solutions and strategies
• Efficient in vivo biotinylated expression system
• Professional data analysis
• Optimize service process to save cost and effort

Alfa Chemistry offers a wide range of biotinylation solutions and focuses on developing highly efficient biotinylation expression systems for a wide range of applications. If you are interested in our services, please contact us immediately.

Reference:

1. Paul. A.S.; et al. A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli. Nucleic Acids Research. 1998, 26(6): 1414-1420.

※ It should be noted that our service is only used for research, not for clinical use.