Antibody Site-specific Biotinylation

One of the strongest interactions between biotin and streptavidin proteins has been widely used in biological research and biotechnology. Labeling or purifying proteins with biotin is an effective method to achieve protein capture, immobilization and functionalization as well as multimerization or bridging molecules. Using the above specific binding, the antibody can be modified and labeled. Biotinylation, the covalent attachment of biotin to biomolecules. Each antibody can be labeled with 3-5 biotins. Immunoconjugates and multispecific antibodies have become efficient research methods, and are important research tools and means for current medical diagnosis and clinical research. Biotin can be attached to a fixed position of an antibody to form a biotinylated antibody, which can be used in the fields of biomarking and diagnosis. Biotinylated antibodies are commonly used reagents in current molecular diagnosis, and recombinant antibodies are monoclonal antibodies, which can be functionally designed. Functionalized single-strand biotinylated fragments can be prepared by simple preparation methods for efficient and sensitive immunoassay applications.

The Services

Alfa Chemistry has a professional antibody site-specific biotinylation solution, and can provide professional customized services for different samples. Our researchers have many years of experience in the field of antibody biotinylation, and the related services we can provide include but are not limited to:

• Construction of expression vector
• Optimization of biotin concentration
• TSH immunoassay
• Antibody site-specific solutions

Antibody site-specific biotinylation

Biotinylation is widely used in the field of biotechnology. The activated biotin can covalently bind to the target protein without affecting the biological activity. Researchers have successfully developed a variety of precise methods that can be used for biotinylation in vivo or in vitro, with the following advantages:

• Controlled site-specific biotinylation
• Little variance between batches
• Little effect on antibody function
• Grooming and Marking Uniformity

Precautions

• Biotin can bind to antibody for specific site modification and labeling
• In order to reduce the influence of steric hindrance, a cross-linked arm-like structure can be added between biotin and the labeled substance
• When modifying or labeling with biotin, it needs to be mixed in proportion

Alfa Chemistry can provide complete solutions, serve a wide range of customers, and provide high-quality antibody biotinylation services. If you are interested in our services, please contact us immediately.

References:

1. Benjamin. M. H.; et al. Site-specific coupling and sterically controlled formation of multimeric antibody Fab fragments with unnatural amino acids. Jmb. 2011, 406(4): 595-603.
2. Michael. F.; et al. Site-specific biotinylation of purified proteins using BirA. Methods Mol Biol. 2015, 1266: 171-84.

※ It should be noted that our service is only used for research, not for clinical use.