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Biotinylated polyethylene glycol (Biotin-PEG) is a versatile bifunctional compound that combines the high-affinity binding capability of biotin with the solubility, flexibility, and biocompatibility of polyethylene glycol (PEG). This hybrid structure enables site-specific, stable conjugation to a wide range of biological molecules, making it an indispensable tool in drug delivery, molecular imaging, diagnostics, and nanotechnology.
At Alfa Chemistry, we offer a broad portfolio of biotin-PEG derivatives tailored for research and development needs across life sciences, materials science, and bioengineering.
Biotin-PEG is typically composed of three modular elements:
The synthesis of biotin-PEG typically involves
1. Biotin Activation
Biotin is activated using coupling agents such as N-hydroxysuccinimide (NHS) or EDC/NHS chemistry, yielding reactive esters for efficient attachment to PEG.
2. PEGylation Reaction
Activated biotin reacts with amine-, thiol-, or hydroxyl-terminated PEG chains, forming stable amide or ester linkages. The process can be customized based on:
3. Functional Tailoring
Post-PEGylation, additional groups (e.g., N3 for click chemistry, maleimide for thiol coupling) can be introduced to expand application versatility.
4. Purification & Quality Control
High-purity biotin-PEG derivatives are obtained via HPLC, dialysis, and mass spectrometry, with optimized pH, temperature, and stoichiometry to maximize yield and activity.
Fig.1 Synthesis scheme of PLGA-PEG-biotin. The process consists of the activation of carboxylate groups of biotin molecules with DCC and NHS. The conjugation of biotin-NHS to PEG-bis-amine and then the conjugation of PEG-biotin to activated PLGA[1].
Biotin-PEG serves as a versatile and powerful tool in numerous biomedical and biochemical applications. Its unique combination of biotin's strong affinity to avidin/streptavidin and PEG's solubility, flexibility, and biocompatibility enables it to act as a modular linker and surface modifier in a wide array of research and development platforms. This section details the primary scientific applications of biotin-PEG.
Researchers often use biotin-PEG for active targeting within drug delivery systems, which focus particularly on cancer treatment applications. The terminal biotin group allows for specific attachment to carriers or receptors that have been functionalized with avidin or streptavidin, allowing for:
a) Target-specific accumulation of therapeutic payloads at diseased sites (e.g., tumors overexpressing biotin receptors)
b) Reduced off-target toxicity and prolonged systemic circulation
c) PEGylated nanoparticles, liposomes, and micelles modified with biotin-PEG are frequently used to encapsulate and deliver chemotherapeutics, nucleic acids, or proteins.
Moreover, the introduction of cleavable linkers like biotin-PEG-SPDP enables stimuli-responsive drug release that occurs through redox potential changes or enzymatic activity within the tumor microenvironment.
Fig.2 The degradation of the lysosome along with GRP78 inhibition by the Ru-1@TPP-PEG-biotin SAN combination therapy is suggested as a new co-targeting cancer treatment[2].
Antibodies, enzymes, oligonucleotides, polymers, nanoparticles, and other substances can be biotin-labeled using biotin-PEG as a biocoupling reagent. The biotin marker facilitates high-affinity capture or detection with either affinity or streptavidin-labeled probes. Applications include:
PEG spacers minimize spatial resistance and non-specific binding, thereby preserving biomolecular function and assay sensitivity.
Biotin-PEG plays an essential role when attaching biomolecules to biosensor surfaces, which consist of gold, silica, or polymer-coated layers. The high specificity and stability of the biotin-streptavidin system provide:
a) Stable and oriented binding of capture molecules (e.g., antibodies, aptamers)
b) Minimized background signal due to PEG's anti-fouling properties
c) Reusability and signal amplification
The most common sensor types are electrochemical biosensors along with quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) sensors, as well as optical and fluorescent sensors.
Fig.3 Preparation of a HRP-DEB biosensor. Functionalization of gold surface with bis(DTPA)-hexynyl and mercaptopropanol; biotin grafting by click reaction followed by streptavidin binding[3].
Biotin-PEG is extensively used to functionalize the surface of nanoparticles, quantum dots, carbon nanotubes, liposomes, and magnetic beads. It allows:
a) Site-specific biotin functionalization for downstream conjugation
b) Enhanced colloidal stability and dispersibility in aqueous systems
c) Reduced protein adsorption and immunogenicity
Typical examples include:
Biotin-PEG derivatives are used to label or modify cell surfaces with biotin groups, enabling interaction with streptavidin-functionalized surfaces or particles for:
a) Cell sorting and capture (e.g., CTC isolation)
b) Live cell imaging and tracking
c) Artificial cell-cell interaction studies
Additionally, biotin-PEG-lipids can be inserted into lipid bilayers of live cells or vesicles, enabling membrane engineering for biosensing and cellular interface design.
Biotin-PEG serves as an indispensable resource in both vaccine conjugate development and diagnostic platform creation, where its functions encompass:
a) Multivalent antigen presentation
b) Spacer molecule for epitope exposure
c) Signal amplification through avidin-enzyme conjugates
The PEG linker secures appropriate spatial orientation while preserving immunogenicity and analytical sensitivity.
Fig.4 Depiction of self-assembled vaccine structures. (A) Structure of MtbHSP70-avidin (MAV) and biotinylated antigens. (B) Structures of OVA peptides for SAV-OVA studies described in Table 2A. (C) and (D) Structures of short (C) and long (D) Flu peptides described in Table 2B for SAV1 and SAV2 used in the Flu study[4].
In summary, biotin-PEG functions as a key enabling reagent for applications that require precise targeting capabilities combined with adaptable structures and biocompatible properties. The capability of this system to connect biomolecules or materials using a non-immunogenic hydrophilic spacer establishes the foundation for next-generation technologies in drug delivery, bioassays, biosensors, and nanomedicine.
Alfa Chemistry offers a comprehensive catalog of high-quality Biotin-PEG derivatives designed for a wide range of applications:
Product Type | Example | Application |
Linear PEG | Biotin-PEG-NH2, Biotin-PEG-SH | Protein conjugation, nanoparticle attachment |
Branched/Multivalent PEG | Biotin-PEG-4-arm | Crosslinking, multivalent binding |
Reactive Esters | Biotin-PEG-NHS | Amide bond formation with primary amines |
Click Chemistry Ready | Biotin-PEG-N3, Biotin-PEG-DBCO | Copper-free or CuAAC ligations |
Fluorescently Labeled | AF647-Biotin-PEG | Live cell imaging, biosensing |
High Molecular Weight | Biotin-PEG-4000, Biotin-PEG-6000 | Long-circulating drug carriers, hydrogels |
Specialty Derivatives | Biotin-PEG-SPDP, Biotin-PEG-Maleimide | Redox-sensitive linkers, thiol-reactive surfaces |
Please feel free to purchase our products:
Catalog Number | Product Name | Inquiry |
BR00028224 | Biotin-PEG- Azide, Biotin-PEG-N3 | Inquiry |
BR00028249 | Biotin-PEG-OH, MW 1k | Inquiry |
BR00028250 | Biotin-PEG-OH, MW 2k | Inquiry |
BR00028251 | Biotin-PEG-OH, MW 3.4k | Inquiry |
BR00028252 | Biotin-PEG-OH, MW 5k | Inquiry |
BR00028253 | Biotin-PEG-OH, MW 10k | Inquiry |
BR00028277 | Biotin-PEG-SH, MW 1k | Inquiry |
BR00028278 | Biotin-PEG-SH, MW 2k | Inquiry |
BR00028279 | Biotin-PEG-SH, MW 3.4k | Inquiry |
BR00028280 | Biotin-PEG-SH, MW 5k | Inquiry |
BR00028281 | Biotin-PEG-SH, MW 10k | Inquiry |
BR00028442 | DBCO-PEG4-Biotin | Inquiry |
BR00028387 | Biotin-PEG8-DBCO | Inquiry |
BR00028234 | 4-Arm PEG-Biotin, MW 2k | Inquiry |
BR00028235 | 4-Arm PEG-Biotin, MW 5k | Inquiry |
BR00028236 | 4-Arm PEG-Biotin, MW 10k | Inquiry |
BR00028237 | 4-Arm PEG-Biotin, MW 20k | Inquiry |
For technical support, product inquiry, or custom synthesis of biotin-PEG derivatives, please contact Alfa Chemistry today and let our scientists help you design the ideal bioconjugate solution for your application.
References:
You may be interested in:
Avidin and Streptavidin Reagents
Biotinylated Peptides, Ligands, Antibodies and Proteins
Biotinylation Reagents
Custom and Synthesis Biotinylation Services
※ It should be noted that our service is only used for research, not for clinical use.