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Your Complete Guide to Biotinylated Polyethylene Glycol: Applications, Benefits, and Mechanisms

Your Complete Guide to Biotinylated Polyethylene Glycol: Applications, Benefits, and Mechanisms

Your Complete Guide to Biotinylated Polyethylene Glycol: Applications, Benefits, and Mechanisms

What Is Biotinylated Polyethylene Glycol?

Biotinylated polyethylene glycol (Biotin-PEG) is a versatile bifunctional compound that combines the high-affinity binding capability of biotin with the solubility, flexibility, and biocompatibility of polyethylene glycol (PEG). This hybrid structure enables site-specific, stable conjugation to a wide range of biological molecules, making it an indispensable tool in drug delivery, molecular imaging, diagnostics, and nanotechnology.

At Alfa Chemistry, we offer a broad portfolio of biotin-PEG derivatives tailored for research and development needs across life sciences, materials science, and bioengineering.

How Is Biotin PEG Structured?

Biotin-PEG is typically composed of three modular elements:

  • Biotin Moiety: A naturally occurring vitamin (B7/H) with a tetrahydrothiophene ring structure, known for its extraordinarily strong and specific binding to avidin or streptavidin (Kd ≈ 10-15 M).
  • Polyethylene Glycol (PEG): A linear or branched hydrophilic polymer, denoted as -(CH2CH2O)n-, available in various molecular weights (e.g., 1k, 2k, 5k Da), enabling precise control over solubility, pharmacokinetics, and steric hindrance.
  • Terminal Functional Groups: Diverse reactive handles such as -NH2, -COOH, -SH, -N3, maleimide, and aldehydes enable specific covalent interactions with other biomolecules or nanostructures.

Biotin PEG Structure.

How Is Biotin PEG Synthesized?

The synthesis of biotin-PEG typically involves

1. Biotin Activation

Biotin is activated using coupling agents such as N-hydroxysuccinimide (NHS) or EDC/NHS chemistry, yielding reactive esters for efficient attachment to PEG.

2. PEGylation Reaction

Activated biotin reacts with amine-, thiol-, or hydroxyl-terminated PEG chains, forming stable amide or ester linkages. The process can be customized based on:

  • PEG architecture (linear, branched, multi-arm)
  • Chain length (molecular weight)
  • Desired terminal functionalities

3. Functional Tailoring

Post-PEGylation, additional groups (e.g., N3 for click chemistry, maleimide for thiol coupling) can be introduced to expand application versatility.

4. Purification & Quality Control

High-purity biotin-PEG derivatives are obtained via HPLC, dialysis, and mass spectrometry, with optimized pH, temperature, and stoichiometry to maximize yield and activity.

Synthesis scheme of PLGA-PEG-biotin.Fig.1 Synthesis scheme of PLGA-PEG-biotin. The process consists of the activation of carboxylate groups of biotin molecules with DCC and NHS. The conjugation of biotin-NHS to PEG-bis-amine and then the conjugation of PEG-biotin to activated PLGA[1].

How Is Biotin-PEG Used in Scientific Applications?

Biotin-PEG serves as a versatile and powerful tool in numerous biomedical and biochemical applications. Its unique combination of biotin's strong affinity to avidin/streptavidin and PEG's solubility, flexibility, and biocompatibility enables it to act as a modular linker and surface modifier in a wide array of research and development platforms. This section details the primary scientific applications of biotin-PEG.

  • Targeted Drug Delivery and Controlled Release

Researchers often use biotin-PEG for active targeting within drug delivery systems, which focus particularly on cancer treatment applications. The terminal biotin group allows for specific attachment to carriers or receptors that have been functionalized with avidin or streptavidin, allowing for:

a) Target-specific accumulation of therapeutic payloads at diseased sites (e.g., tumors overexpressing biotin receptors)

b) Reduced off-target toxicity and prolonged systemic circulation

c) PEGylated nanoparticles, liposomes, and micelles modified with biotin-PEG are frequently used to encapsulate and deliver chemotherapeutics, nucleic acids, or proteins.

Moreover, the introduction of cleavable linkers like biotin-PEG-SPDP enables stimuli-responsive drug release that occurs through redox potential changes or enzymatic activity within the tumor microenvironment.

Ru-1@TPP-PEG-biotin SAN combination therapy degrades lysosomes and inhibits GRP78Fig.2 The degradation of the lysosome along with GRP78 inhibition by the Ru-1@TPP-PEG-biotin SAN combination therapy is suggested as a new co-targeting cancer treatment[2].

  • Bioconjugation and Molecular Labeling

Antibodies, enzymes, oligonucleotides, polymers, nanoparticles, and other substances can be biotin-labeled using biotin-PEG as a biocoupling reagent. The biotin marker facilitates high-affinity capture or detection with either affinity or streptavidin-labeled probes. Applications include:

  • Western blotting
  • ELISA
  • FRET assays
  • Single molecule tracing

PEG spacers minimize spatial resistance and non-specific binding, thereby preserving biomolecular function and assay sensitivity.

  • Biosensor Functionalization

Biotin-PEG plays an essential role when attaching biomolecules to biosensor surfaces, which consist of gold, silica, or polymer-coated layers. The high specificity and stability of the biotin-streptavidin system provide:

a) Stable and oriented binding of capture molecules (e.g., antibodies, aptamers)

b) Minimized background signal due to PEG's anti-fouling properties

c) Reusability and signal amplification

The most common sensor types are electrochemical biosensors along with quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) sensors, as well as optical and fluorescent sensors.

Preparation of a HRP-DEB biosensor.Fig.3 Preparation of a HRP-DEB biosensor. Functionalization of gold surface with bis(DTPA)-hexynyl and mercaptopropanol; biotin grafting by click reaction followed by streptavidin binding[3].

  • Nanoparticle and Surface Modification

Biotin-PEG is extensively used to functionalize the surface of nanoparticles, quantum dots, carbon nanotubes, liposomes, and magnetic beads. It allows:

a) Site-specific biotin functionalization for downstream conjugation

b) Enhanced colloidal stability and dispersibility in aqueous systems

c) Reduced protein adsorption and immunogenicity

Typical examples include:

  • Biotin-PEG-gold nanoparticles for targeted photothermal therapy
  • Magnetic biotin-PEG beads for cell separation or RNA pull-down
  • Biotinylated PEG-hydrogels for bioactive scaffold development
  • Cell Capture, Tracking, and Surface Engineering

Biotin-PEG derivatives are used to label or modify cell surfaces with biotin groups, enabling interaction with streptavidin-functionalized surfaces or particles for:

a) Cell sorting and capture (e.g., CTC isolation)

b) Live cell imaging and tracking

c) Artificial cell-cell interaction studies

Additionally, biotin-PEG-lipids can be inserted into lipid bilayers of live cells or vesicles, enabling membrane engineering for biosensing and cellular interface design.

  • Vaccine and Diagnostic Conjugates

Biotin-PEG serves as an indispensable resource in both vaccine conjugate development and diagnostic platform creation, where its functions encompass:

a) Multivalent antigen presentation

b) Spacer molecule for epitope exposure

c) Signal amplification through avidin-enzyme conjugates

The PEG linker secures appropriate spatial orientation while preserving immunogenicity and analytical sensitivity.

Depiction of self-assembled vaccine structures.Fig.4 Depiction of self-assembled vaccine structures. (A) Structure of MtbHSP70-avidin (MAV) and biotinylated antigens. (B) Structures of OVA peptides for SAV-OVA studies described in Table 2A. (C) and (D) Structures of short (C) and long (D) Flu peptides described in Table 2B for SAV1 and SAV2 used in the Flu study[4].

In summary, biotin-PEG functions as a key enabling reagent for applications that require precise targeting capabilities combined with adaptable structures and biocompatible properties. The capability of this system to connect biomolecules or materials using a non-immunogenic hydrophilic spacer establishes the foundation for next-generation technologies in drug delivery, bioassays, biosensors, and nanomedicine.

What Types of Biotin-PEG Derivatives are Available at Alfa Chemistry?

Alfa Chemistry offers a comprehensive catalog of high-quality Biotin-PEG derivatives designed for a wide range of applications:

Product Type Example Application
Linear PEGBiotin-PEG-NH2, Biotin-PEG-SHProtein conjugation, nanoparticle attachment
Branched/Multivalent PEGBiotin-PEG-4-armCrosslinking, multivalent binding
Reactive EstersBiotin-PEG-NHSAmide bond formation with primary amines
Click Chemistry ReadyBiotin-PEG-N3, Biotin-PEG-DBCOCopper-free or CuAAC ligations
Fluorescently LabeledAF647-Biotin-PEGLive cell imaging, biosensing
High Molecular WeightBiotin-PEG-4000, Biotin-PEG-6000Long-circulating drug carriers, hydrogels
Specialty DerivativesBiotin-PEG-SPDP, Biotin-PEG-MaleimideRedox-sensitive linkers, thiol-reactive surfaces

Please feel free to purchase our products:

For technical support, product inquiry, or custom synthesis of biotin-PEG derivatives, please contact Alfa Chemistry today and let our scientists help you design the ideal bioconjugate solution for your application.

References:

  1. Mehdizadeh M, et al. (2016). "Biotin decorated PLGA nanoparticles containing SN-38 designed for cancer therapy". Artificial Cells, Nanomedicine, and Biotechnology. 45(3), 1-10.
  2. Purushothaman B, et al. (2020). "Multifunctional TPP-PEG-biotin self-assembled nanoparticle drug delivery-based combination therapeutic approach for co-targeting of GRP78 and lysosome". Journal of Nanobiotechnology. 18, 102.
  3. Yáñez-Sedeño P, et al. (2019). "Copper(I)-Catalyzed Click Chemistry as a Tool for the Functionalization of Nanomaterials and the Preparation of Electrochemical (Bio)Sensors". Sensors. 19(10), 2379.
  4. Leblanc P. R, et al. (2014). “VaxCelerate II: Rapid Development of a Self-Assembling Vaccine for Lassa Fever”. Human Vaccines & Immunotherapeutics. 10(10), e34413.

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