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Catalog | BR00028224 |
Description | Sulfo-NHS-Biotin is a water-soluble reagent that allows simple and efficient biotinylation of antibodies, proteins and other macromolecules containing primary amines. It is soluble in water and non-cleavable, allowing simple and efficient biotinylation of antibodies, proteins and any other macromolecules containing primary amines in solution. |
Application | This kit is mainly used to label primary antibodies, secondary antibodies, soluble MHC/peptide complexes (monomers), recombinant proteins. In biology, biotin-labeled molecules are used for: FC, ELISA Detection, ELISA, WB, IHC-P, ICFC. |
Storage | Store the kit unopened at 2-8℃ for 1 year, and the dissolved biotin can be stored at 2-8℃ for 1 week. |
Component | A: Sulfo-NHS-Biotin, 8 x 2 mg; reaction buffer, 30 mL; Protein concentrator, 8. B: Sulfo-NHS-Biotin, 8 x 2 mg |
Feature | Reacts with primary amines. The sulfo-NHS group increases the water solubility of the reagent compared to ordinary NHS-ester compounds. Labeled biotinylate only labels surface proteins throughout the cell Permanent amide bond; the spacer arm cannot be cleaved It consists only of natural biotin pentanoic acid groups. |
※ It should be noted that our product is only used for research, not for clinical use.