Site Specific Biotinylation Kit

Site Specific Biotinylation Kit

Catalog	BR00028227
Description	The Site Specific Biotinylation Kit is optimized for labeling antibodies at the 100 ug and 1 mg scale. These kits are packaged in convenient single-use microtubes, eliminating the difficulty of weighing small amounts of reagents.
Application	This kit is mainly used to label primary antibodies, secondary antibodies, soluble MHC/peptide complexes (monomers), recombinant proteins. In biology, biotin-labeled molecules are used for: FC, ELISA Detection, ELISA, WB, IHC-P, ICFC.
Storage	Store the kit unopened at 2-8℃ for 1 year, and the dissolved biotin can be stored at 2-8℃ for 1 week.
Component	Biotin Linker (MW ~ 1200); Reaction Buffer pH8; Protein concentrator; DMSO; TCEP.HCl
Feature	Biotinylated antibodies typically maintain their biological activity because the biotin group is relatively small. Conventional dimerization methods, such as using amine-NHS esters or converting amines to thiols followed by maleimide chemistry, result in random biotin attachment on antibodies. In some cases, biotin labeling in specific Fab regions may interfere with antigen binding. However, with site-specific biotinylation chemistry, the biotin linker is placed at the thiol bridge position, ensuring that antibody-antigen binding is not affected. This approach enhances the sensitivity of bioassays like ELISA and Western Blot.
Specification	3x100 ug; 8x100 ug; 1x1 mg; 3x1 mg

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